Interferon alpha 4 Active Protein | IFNA4 active protein

Interferon alpha 4, Recombinant, Mouse (Interferon alpha-4, IFN-alpha-4, Ifna4)

Cat. Num. AAA636497

• Gene Names: IFNA4; INFA4; IFN-alpha4a
• Purity: Highly Purified; ~95% (Ion exchange, hydrophobic interaction and size exclusion chromatography)
• Synonyms: Interferon alpha 4; Recombinant; Mouse (Interferon alpha-4; IFN-alpha-4; Ifna4); IFNA4 active protein

• Purity/Purification: Highly Purified; ~95% (Ion exchange, hydrophobic interaction and size exclusion chromatography)
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA.

• Biological Activity: Determined relative to a lab standard of Mu IFN-alpha A which was calibrated to the NIH Murine IFN-a standard (Ga02-901-511). Mouse (L929/EMCV) in this assay the EC50 for IFN is ~5U/ml.
• Endotoxin Level: ~1EU/ug
• Preparation and Storage: Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(The activity of Mu Alpha-A [E.coli] and Mu Alpha-4 [mammalian] was compared in an L929/EMCV CPE assay. The EC50 for Mu Alpha-A [E.coli] in this experiment was 23.5pg/ml while the EC50 for Mu Alpha-4 [mammalian] was 3.59pg/ml.)

Related Product Information for IFNA4 active protein

Most mammalian species have multiple IFN-a subtypes. Although the reasons for these multiple subtypes are not fully known, there are clear cell type and temporal differences in their expression. A recent study established a nomenclature for the murine IFN-a subtypes (van Pesch et al. 2004) and determined relative activities of the subtypes with protein quantification by phosphorimaging of metabolically-labeled protein. In this study, Mu IFN-a4 was deemed to have average antiviral activity when compared with the potency of the other subtypes. Mu IFN-a4 was initially cloned by Zwarthoff et al. [(1985) Nuc. Acids Res. 13(3) 791], and has been extensively studied. It is apparently expressed early in viral infection in a protein synthesis independent manner, and its expression is induced by phosphorylation of IRF-3. It may be that Mu IFN-a4, like IFN-b, has a priming function on cells, enabling the expression of other Mu IFN-a subtypes [Reviewed by Mesplède et al. (2003) Autoimmunity 36(8):447 and Asselin-Paturel & Trinchieri (2005) J. Exp. Med. 202(4):461]. Thus, this interferon is among the first observed after viral infection. Intriguingly, while this interferon is expressed in a large variety of cell types, one report suggests that the expression level in dendritic cells is low to non-existent [Barchet et al. (2002) J. Exp. Med. 195(4):507]

Product Categories/Family for IFNA4 active protein

Growth Factors; Cytokines; Growth Factors-Interferon

NCBI and Uniprot Product Information

• NCBI GI #: 10835103
• NCBI GeneID: 3441
• NCBI Accession #: NP_066546.1
• NCBI GenBank Nucleotide #: NM_021068.2
• UniProt Accession #: P05014; P13358; Q14CS4; Q5VV15
• Molecular Weight: ~19.5kD
• NCBI Official Full Name: interferon alpha-4
• NCBI Official Synonym Full Names: interferon, alpha 4
• NCBI Official Symbol: IFNA4
• NCBI Official Synonym Symbols: INFA4; IFN-alpha4a
• NCBI Protein Information: interferon alpha-4; IFN-alpha-4; interferon alpha-4B; interferon alpha-76; interferon alpha-M1
• UniProt Protein Name: Interferon alpha-4
• UniProt Gene Name: IFNA4
• UniProt Synonym Gene Names: IFN-alpha-4
• UniProt Entry Name: IFNA4_HUMAN

Uniprot Description

IFNA4: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. Belongs to the alpha/beta interferon family.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 9p22

Cellular Component: extracellular space; extracellular region

Molecular Function: interferon-alpha/beta receptor binding; cytokine activity

Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; response to virus; regulation of MHC class I biosynthetic process; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; B cell differentiation; natural killer cell activation during immune response; innate immune response; blood coagulation; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein