Integrin alphaV, beta8 Active Protein | ITGAV active protein
Integrin alphaV, beta8, Recombinant, Human
Purity
Highly Purified90%, as determined by SDS-PAGE and visualized by silver stain.
Synonyms
Integrin alphaV; beta8; Recombinant; Human; ITGAV active protein
Purity/Purification
Highly Purified
90%, as determined by SDS-PAGE and visualized by silver stain.
90%, as determined by SDS-PAGE and visualized by silver stain.
Form/Format
Supplied as a lyophilized powder in 50mM Tris, 150mM NaCl and 2mM MgCl2, pH 7.4.
Activity
Measured by its ability to bind rhLap. When rhIntegrin V 8 is coated at 0.1ug/ml, rhLap binds with an apparent KD < 0.5 nM.
Endotoxin Level
< 1.0 EU per 1 g of the protein as determined by the LAL method.
Reconstitution
It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
Preparation and Storage
Lyophilized samples are stable for up to twelve months at -20 degree C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2 degree -8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Related Product Information for ITGAV active protein
Integrin v 8 is one of five v integrins and the only known 8 integrin. 1-3 The non-covalent heterodimer of 170 kD v and ~90 kD 8 integrin type I transmembrane glycoprotein subunits is expressed in yolk sac, placenta, brain perivascular astrocytes, Schwann cells, renal glomerular mesangial cells and pulmonary epithelial cells. 3-7 Unlike other v integrins, v 8 does not appear to assume different activation states, and the cytoplasmic tail does not connect to the cytoskeleton. 3, 8 It binds ligands containing an RGD motif, including vitronectin, fibrin and the latency associated peptide (LAP) of the latent TGF- complex. 7-12 High affinity binding of v 8 to LAP allows proteolytic cleavage by MT1-MMP, which releases active TGF-. This mechanism differs from that of v 6, the other v integrin which can activate TGF- from latency through non-proteolytic mechanisms. 13 Downstream effects of TGF- activation include control of cell growth and associated vascularization. 10-13 Deletion of either v or 8 reveals that v 8 is required for vascular morphogenesis in the embryonic brain and yolk sac. 4, 14, 15 The 962 aa human v extracellular domain (ECD) shares 92-95% aa sequence identity with mouse, rat and cow v, while the 642 aa human 8 ECD shares 92%, 92%, 89%, 87% and 87% aa identity with cow, dog, rabbit, mouse and rat 8, respectively. The 8 ECD of 8 shows low (~35%) aa identity with other integrin subunits, and the cytoplasmic tail is unlike any other integrin. The v ECD contains an N-terminal - propeller structure, followed by domains termed thigh, calf-1 and calf-2. 1 The 8 ECD contains a vWFA domain, which interacts with the v -propeller to form a binding domain. Each subunit has a transmembrane sequence and a short cytoplasmic tail.
Product Categories/Family for ITGAV active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Based on N-terminal sequencing, the recombinant human Integrin V subunit starts at Phe 31 and has a calculated molecular mass of approximately 111 kD. The 8 subunit starts at Glu 43 and has a calculated molecular mass of approximately 75.3 kD. As a result
NCBI Official Full Name
integrin alpha-V
NCBI Official Symbol
ITGAV
NCBI Protein Information
integrin alpha-V; alphaV integrin; integrin alpha v; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
Protein Family
Research Articles on ITGAV
Similar Products
Product Notes
The ITGAV (Catalog #AAA637428) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Integrin alphaV, beta8, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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