CRIM1 active protein

CRIM1, Recombinant, Human (Cysteine-rich motor neuron 1 protein)

Cat. Num. AAA635530

• Gene Names: CRIM1; S52; CRIM-1
• Purity: Purified; 90% (SDS-PAGE)
• Synonyms: CRIM1; Recombinant; Human (Cysteine-rich motor neuron 1 protein); CRIM1 active protein

• Purity/Purification: Purified; 90% (SDS-PAGE)
• Form/Format: Supplied as a lyophilized powder from PBS. Carrier free. Reconstitute with 100ug/ml PBS.

• Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of NIH/3T3 mouse embryonic fibroblasts. CRIM1 immobilized at 3ug/ml, 100ul/well, will mediate >50% NIH3T3 cell adhesion (added at 1x10e5 cells/well).
• Endotoxin Level: 1EU/ug (LAL)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute with 100ug/ml PBS. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for CRIM1 active protein

Cysteine rich motor neuron 1 (CRIM1) is a type I transmembrane glycoprotein of the chordin-like cysteine-rich repeat (CRR) family of BMP inhibitors. The ~130kD, 1036aa CRIM1 contains a 34aa signal sequence, a 905aa extracellular domain (ECD), a 21aa transmembrane domain and a 76aa cytoplasmic domain. The ECD includes an N-terminal IGF-binding protein-like motif and six chordin-like von Willebrand C-type CRRs. The ECD can be released from the cell, presumably by proteolytic processing. Human CRIM1 ECD shows 88%, 88%, 91%, 86%, 87%, 83% and 72% aa identity with mouse, rat, dog, cow, opossum, chick and zebrafish CRIM1 ECD, respectively. CRIM1 can interact with TGF-beta family ligands, including BMPs 2, 4 and 7, via its CRR domains. It binds BMPs intracellularly and antagonizes them by lowering their expression, processing and secretion. CRIM1 is expressed in the developing spinal cord in the floor plate and developing motor neurons. It is also expressed by perivascular smooth muscle cells and aligns at points of cell-cell contact during endothelial cell capillary formation. Endothelial cell expression in vitro appears to be specific to cells that are adherent and growing. CRIM1 is also expressed in a spatially and temporally restricted manner in the developing lens, limbs, kidney, teeth and testis. Studies where CRIM1 expression is manipulated in developing mouse, chick and zebrafish support its involvement in regulation of vascular and somitic development and organogenesis.

Product Categories/Family for CRIM1 active protein

Molecular Biology; MB-Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 10092639
• NCBI GeneID: 51232
• NCBI Accession #: NP_057525.1
• NCBI GenBank Nucleotide #: NM_016441.2
• UniProt Accession #: Q9NZV1; Q2M2G4; Q59GH0; Q7LCQ5; Q9H318
• Molecular Weight: 125-140kD
• NCBI Official Full Name: cysteine-rich motor neuron 1 protein
• NCBI Official Synonym Full Names: cysteine rich transmembrane BMP regulator 1 (chordin-like)
• NCBI Official Symbol: CRIM1
• NCBI Official Synonym Symbols: S52; CRIM-1
• NCBI Protein Information: cysteine-rich motor neuron 1 protein; cysteine-rich repeat-containing protein S52
• UniProt Protein Name: Cysteine-rich motor neuron 1 protein
• Protein Family: Cysteine-rich motor neuron 1 protein
• UniProt Gene Name: CRIM1
• UniProt Synonym Gene Names: S52; CRIM-1
• UniProt Entry Name: CRIM1_HUMAN

NCBI Description

This gene encodes a transmembrane protein containing six cysteine-rich repeat domains and an insulin-like growth factor-binding domain. The encoded protein may play a role in tissue development though interactions with members of the transforming growth factor beta family, such as bone morphogenetic proteins. [provided by RefSeq, Nov 2010]

Uniprot Description

CRIM1: May play a role in CNS development by interacting with growth factors implicated in motor neuron differentiation and survival. May play a role in capillary formation and maintenance during angiogenesis. Modulates BMP activity by affecting its processing and delivery to the cell surface.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: plasma membrane; integral to membrane

Molecular Function: serine-type endopeptidase inhibitor activity; insulin-like growth factor binding; insulin-like growth factor receptor activity; PDZ domain binding

Biological Process: nervous system development; insulin-like growth factor receptor signaling pathway; regulation of cell growth