Acetylcholinesterase (AchE) Recombinant Protein | AchE recombinant protein

Recombinant Acetylcholinesterase (AchE)

Cat. Num. AAA2010961

• Gene Names: Ache; mE1a; mE1b; mE1c; mE1d; mE1e; mE1d'; mE1c-long
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Acetylcholinesterase (AchE); Recombinant Acetylcholinesterase (AchE); AchE recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-SYFLVYGVPG FSKDNESLIS RAQFLAGVRI GVPQASDLAA EAVVLHYTDW LHPEDPTHLR DAMSAVVGDH NVVCPVAQLA GRLAAQGARV YAYIFEHRAS TLTWPLWMGV PHGYEIEFIF GLPLDPSLNY TTEERIFAQR LMKYWTNFAR TGDPNDPRDS KSPQWPPYTT AAQQYVSLNL KPL
• Sequence Length: 614

• Applicable Applications for AchE recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ser367~Leu549 (Accession # P21836) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 13928664
• NCBI GeneID: 11423
• NCBI Accession #: NP_033729.1
• NCBI GenBank Nucleotide #: NM_009599.3
• UniProt Accession #: P21836
• Molecular Weight: 22.1kDa
• NCBI Official Full Name: acetylcholinesterase
• NCBI Official Synonym Full Names: acetylcholinesterase
• NCBI Official Symbol: Ache
• NCBI Official Synonym Symbols: mE1a; mE1b; mE1c; mE1d; mE1e; mE1d'; mE1c-long
• NCBI Protein Information: acetylcholinesterase
• UniProt Protein Name: Acetylcholinesterase
• Protein Family: Acetylcholinesterase
• UniProt Gene Name: Ache
• UniProt Synonym Gene Names: AChE
• UniProt Entry Name: ACES_MOUSE

Uniprot Description

ACHE: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Belongs to the type-B carboxylesterase/lipase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerophospholipid; Membrane protein, GPI anchor; EC 3.1.1.7; Hydrolase

Cellular Component: Golgi apparatus; extracellular space; neuron projection; cell surface; rough endoplasmic reticulum; endoplasmic reticulum lumen; dendrite; extracellular region; nuclear envelope; presynaptic membrane; postsynaptic membrane; membrane; cell soma; axon; perinuclear region of cytoplasm; cytoplasm; plasma membrane; basal lamina; synapse; cell junction; neuromuscular junction

Molecular Function: collagen binding; choline binding; serine hydrolase activity; protein binding; protein homodimerization activity; protein self-association; cholinesterase activity; hydrolase activity; laminin binding; acetylcholinesterase activity; acetylcholine binding

Biological Process: choline metabolic process; neurotransmitter catabolic process; synaptogenesis; acetylcholine metabolic process; retina development in camera-type eye; receptor internalization; neurotransmitter receptor biosynthetic process; acetylcholine catabolic process; regulation of receptor recycling; positive regulation of axonogenesis; positive regulation of dendrite morphogenesis; cell adhesion; protein tetramerization