Testing Data
(Figure 1. Absorption spectrum of WST-8 formazan Figure 1 shows the absorption spectrum of WST-8 formazan. Since the absorbance at 460 nm is proportional to the number of viable cells in the medium, the viable cell number can be determined using the absorbance of a previously prepared calibration curve.)
Cell Counting Kit-8 Accessory Reagent
Cell Counting Kit-8 (CCK-8)
Synonyms
Cell Counting Kit-8; Cell Counting Kit-8 (CCK-8); Cell Counting Kit-8 accessory reagent
Preparation and Storage
Store at 4 degree C in dark for one year.
Testing Data
(Figure 1. Absorption spectrum of WST-8 formazan Figure 1 shows the absorption spectrum of WST-8 formazan. Since the absorbance at 460 nm is proportional to the number of viable cells in the medium, the viable cell number can be determined using the absorbance of a previously prepared calibration curve.)
Testing Data
(Figure 1. Absorption spectrum of WST-8 formazan Figure 1 shows the absorption spectrum of WST-8 formazan. Since the absorbance at 460 nm is proportional to the number of viable cells in the medium, the viable cell number can be determined using the absorbance of a previously prepared calibration curve.)
Testing Data
(Figure 2. Cell proliferation assay using CCK-8 Culture medium: MEM, 10% FBSIncubation: 37 ËšC, 5% CO2, 2 hoursDeteactin: 450nm)
Testing Data
(Figure 2. Cell proliferation assay using CCK-8 Culture medium: MEM, 10% FBSIncubation: 37 ËšC, 5% CO2, 2 hoursDeteactin: 450nm)
Testing Data
(Figure 3. Toxicological test of chemicals using CCK-8 Cell line: helaMedium: DMEM, 10% FBS Chemicals: 200 uM Cisplatin (DDP)Incubation: 37 degree C, 5% CO2, 2 hours)
Testing Data
(Figure 3. Toxicological test of chemicals using CCK-8 Cell line: helaMedium: DMEM, 10% FBS Chemicals: 200 uM Cisplatin (DDP)Incubation: 37 degree C, 5% CO2, 2 hours)
Related Product Information for Cell Counting Kit-8 accessory reagent
Background/Introduction: Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt. WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium, monosodium salt]* produces a water-soluble formazan dye upon reduction in the presence of an electron carrier. Cell Counting Kit-8 is a one-bottle solution; no premixing of components is required. Cell Counting Kit-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by the activity of dehydrogenases in cells is directly proportional to the number of living cells. The cell proliferation assay using CCK-8 correlates well with the [(3)H]thymidine incorporation assay. And the CCK-8 assay can also be substituted for the [(3)H]thymidine incorporation assay. The detection sensitivity of CCK-8 is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.
Product Categories/Family for Cell Counting Kit-8 accessory reagent
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Product Notes
The Cell Counting Kit-8 (Catalog #AAA176412) is an Accessory Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Cell Counting Kit-8, Accessory Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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