CD34 recombinant protein

CD34 Protein, Rat, Recombinant (hFc Tag)

Cat. Num. AAA8123405

• Purity: >80% as determined by SDS-PAGE
• Synonyms: CD34; CD34 Protein; Rat; Recombinant (hFc Tag); Rat CD34 Protein (Fc Tag); CD34 molecule; CD34 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr291

• Species: Rat
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 37
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the rat CD34 (B1PLB1)(Met1-Thr291) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD34 recombinant protein

Background: Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias.

Product Categories/Family for CD34 recombinant protein

Cardiac Stem Cell Markers Hemangioblast Markers

References

Hogan CJ, et al. (2002) Differential long-term and multilineage engraftment potential from subfractions of human CD34+ cord blood cells transplanted into NOD/SCID mice. Proc Nat Acad Sci USA. 99 (1): 413-8.Nielsen JS,et al. (2009) CD34 is a key regulator of hematopoietic stem cell trafficking to bone marrow and mast cell progenitor trafficking in the periphery. Microcirculation. 16(6): 487-96.Mastrandrea F,et al. (2009) CD34+ hemopoietic precursor and stem cells traffic in peripheral blood of celiac patients is significantly increased but not directly related to epithelial damage severity. Eur Ann Allergy Clin Immunol. 40(3): 90-103.Pasquet S,et al. (2009) Long-term benefit of intracardiac delivery of autologous granulocyte-colony-stimulating factor-mobilized blood CD34+ cells containing cardiac progenitors on regional heart structure and function after myocardial infarct. Cytotherapy. 11(8): 1002-15.

NCBI and Uniprot Product Information

• NCBI GI #: 157821377
• NCBI GeneID: 305081
• NCBI Accession #: NP_001100672.1
• NCBI GenBank Nucleotide #: NM_001107202.2
• UniProt Accession #: B1PLB1
• Molecular Weight: 41,239 Da
• NCBI Official Full Name: hematopoietic progenitor cell antigen CD34
• NCBI Official Synonym Full Names: CD34 molecule
• NCBI Official Symbol: Cd34
• NCBI Protein Information: hematopoietic progenitor cell antigen CD34
• UniProt Protein Name: CD34 antigen (Predicted)
• Protein Family: Hematopoietic progenitor cell antigen
• UniProt Gene Name: Cd34

Uniprot Description

CD34: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. Belongs to the CD34 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q27

Cellular Component: apical plasma membrane; basal plasma membrane; cell surface; cytoplasm; external side of plasma membrane; extracellular region; integral to plasma membrane; intercellular bridge; lysosome; membrane; perinuclear region of cytoplasm; plasma membrane

Molecular Function: carbohydrate binding; sulfate binding; transcription factor binding

Biological Process: cell motility involved in cell locomotion; cell proliferation; cell-cell adhesion; endothelial cell proliferation; glomerular filtration; hemopoiesis; leukocyte migration; negative regulation of blood coagulation; negative regulation of nitric oxide biosynthetic process; negative regulation of tumor necrosis factor production; positive regulation of angiogenesis; positive regulation of interleukin-10 production; positive regulation of odontogenesis; regulation of blood pressure; signal transduction; tissue homeostasis