Mouse amiloride binding protein 1 (amine oxidase (copper-containing) ) ELISA Kit | ABP1 elisa kit

Mouse diamine oxidase, DAO ELISA Kit

Cat. Num. AAA705118

• Gene Names: Aoc1; DAO; Abp1; 1600012D06Rik
• Reactivity: Mouse
• Synonyms: amiloride binding protein 1 (amine oxidase (copper-containing) ); Mouse diamine oxidase; DAO ELISA Kit; ABP; AOC1; DAO; DAO1; KAO; amiloride binding protein 1; amiloride-binding protein; amiloride-binding protein-1; amiloride-sensitive amine oxidase; diamine oxidase; histaminase; kidney amine oxidase; ABP1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse DAO. No significant cross-reactivity or interference between mouse DAO and analogues was observed.
• Sequence Length: 751

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 15.6 mU/ml -1000 mU/ml.
• Sensitivity: The minimum detectable dose of mouse DAO is typically less than 3.9 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABP1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DAO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DAO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DAO is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DAO bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 47605364
• NCBI GeneID: 76507
• UniProt Accession #: Q8JZQ5; Q8R229; Q8VC36
• Molecular Weight: 85,424 Da
• NCBI Official Full Name: Amiloride-sensitive amine oxidase
• NCBI Official Synonym Full Names: amine oxidase, copper-containing 1
• NCBI Official Symbol: Aoc1
• NCBI Official Synonym Symbols: DAO; Abp1; 1600012D06Rik
• NCBI Protein Information: amiloride-sensitive amine oxidase [copper-containing]; amiloride-sensitive amine oxidase [copper-containing]; ABP; histaminase; diamine oxidase; amiloride-binding protein 1; amine oxidase copper domain-containing protein 1; amiloride binding protein 1 (amine oxidase, copper-containing)
• UniProt Protein Name: Amiloride-sensitive amine oxidase [copper-containing]
• Protein Family: ARS-binding protein
• UniProt Gene Name: Aoc1
• UniProt Synonym Gene Names: Abp1; DAO; Diamine oxidase
• UniProt Entry Name: AOC1_MOUSE

Uniprot Description

Function: Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis

Catalytic activity: Histamine + H2O + O2 = (imidazol-4-yl)acetaldehyde + NH3 + H2O2.

Cofactor: Binds 1 copper ion per subunit