Human chaperonin containing TCP1, subunit 5 (epsilon) ELISA Kit | CCT5 elisa kit

Human T-complex protein 1 subunit epsilon, CCT5 ELISA Kit

Cat. Num. AAA930379

• Gene Names: CCT5; CCTE; HEL-S-69; CCT-epsilon; TCP-1-epsilon
• Reactivity: Human
• Synonyms: chaperonin containing TCP1; subunit 5 (epsilon); Human T-complex protein 1 subunit epsilon; CCT5 ELISA Kit; Human T-complex protein 1 subunit epsilon (CCT5) ELISA kit; CCT-epsilon; CCTE; KIAA0098; TCP-1-epsilon; T-complex protein 1; epsilon subunit; CCT5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CCT5. No significant cross-reactivity or interference between human CCT5 and analogues was observed.
• Sequence Length: 541

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 15.6 pg/ml-1000 pg/ml.
• Sensitivity: The minimum detectable dose of human CCT5 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCT5 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CCT5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCT5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCT5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCT5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 24307939
• NCBI GeneID: 22948
• NCBI Accession #: NP_036205.1
• NCBI GenBank Nucleotide #: NM_012073.3
• UniProt Accession #: P48643; A8JZY8; A8K2X8; B4DYD8
• Molecular Weight: 59,671 Da
• NCBI Official Full Name: T-complex protein 1 subunit epsilon
• NCBI Official Synonym Full Names: chaperonin containing TCP1, subunit 5 (epsilon)
• NCBI Official Symbol: CCT5
• NCBI Official Synonym Symbols: CCTE; HEL-S-69; CCT-epsilon; TCP-1-epsilon
• NCBI Protein Information: T-complex protein 1 subunit epsilon; epididymis secretory protein Li 69; T-complex protein 1, epsilon subunit
• UniProt Protein Name: T-complex protein 1 subunit epsilon
• Protein Family: T-complex protein
• UniProt Gene Name: CCT5
• UniProt Synonym Gene Names: CCTE; KIAA0098; TCP-1-epsilon
• UniProt Entry Name: TCPE_HUMAN

NCBI Description

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. In addition, three pseudogenes of this gene have been identified. [provided by RefSeq, Jun 2010]

Uniprot Description

CCT-epsilon: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Defects in CCT5 are the cause of hereditary sensory neuropathy autosomal recessive with spastic paraplegia (HSNSP). The disease is characterized by spastic paraplegia and progressive distal sensory neuropathy leading to mutilating ulcerations of the upper and lower limbs. Belongs to the TCP-1 chaperonin family.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: chaperonin-containing T-complex; microtubule; centrosome; cytoplasm; nucleolus; cytosol

Molecular Function: protein binding; unfolded protein binding; beta-tubulin binding; G-protein beta-subunit binding; ATP binding

Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; binding of sperm to zona pellucida; response to virus

Disease: Neuropathy, Hereditary Sensory, With Spastic Paraplegia, Autosomal Recessive