Rabbit ATP Binding Cassette Transporter G1 ELISA Kit | ABCG1 elisa kit

Rabbit ATP Binding Cassette Transporter G1 ELISA Kit

Cat. Num. AAA733315

• Gene Names: ABCG1; ABC8; WHITE1
• Reactivity: Rabbit
• Synonyms: ATP Binding Cassette Transporter G1; Rabbit ATP Binding Cassette Transporter G1 ELISA Kit; ABCG1 elisa kit

• Reactivity: Rabbit

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This ABCG1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit ABCG1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABCG1 elisa kit

Principle of the assay: ABCG1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ABCG1. Standards or samples are then added to the microtiter plate wells and ABCG1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ABCG1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ABCG1 are added to each well to “sandwich” the ABCG1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ABCG1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ABCG1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ABCG1 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 16304311
• NCBI GeneID: 9619
• Molecular Weight: 86,628 Da
• NCBI Official Full Name: ATP-binding cassette transporter G1
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family G (WHITE), member 1
• NCBI Official Symbol: ABCG1
• NCBI Official Synonym Symbols: ABC8; WHITE1
• NCBI Protein Information: ATP-binding cassette sub-family G member 1; ABC transporter 8; homolog of Drosophila white; ATP-binding cassette transporter 8; ATP-binding cassette transporter member 1 of subfamily G; white protein homolog (ATP-binding cassette transporter 8)
• UniProt Protein Name: ATP-binding cassette sub-family G member 1
• Protein Family: ABC transporter G family
• UniProt Gene Name: ABCG1
• UniProt Synonym Gene Names: ABC8; WHT1
• UniProt Entry Name: ABCG1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. Six alternative splice variants have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCG1: Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter, ABC family; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: nucleoplasm; Golgi membrane; Golgi apparatus; recycling endosome; endoplasmic reticulum membrane; intracellular membrane-bound organelle; mitochondrion; integral to plasma membrane; plasma membrane; endosome; external side of plasma membrane

Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; phospholipid transporter activity; protein heterodimerization activity; cholesterol transporter activity; toxin transporter activity; cholesterol binding; phospholipid binding; sterol-transporting ATPase activity; ADP binding; ATP binding

Biological Process: cholesterol metabolic process; response to high density lipoprotein stimulus; detection of hormone stimulus; positive regulation of cholesterol biosynthetic process; intracellular cholesterol transport; cholesterol efflux; lipoprotein metabolic process; response to lipid; response to organic substance; amyloid precursor protein catabolic process; cholesterol homeostasis; reverse cholesterol transport; regulation of transcription, DNA-dependent; phospholipid efflux; transmembrane transport; phospholipid homeostasis