Human ATP Binding Cassette Transporter G1 ELISA Kit | ABCG1 elisa kit

Human ATP Binding Cassette Transporter G1 ELISA Kit

Cat. Num. AAA760294

• Gene Names: ABCG1; ABC8; WHITE1
• Reactivity: Human
• Synonyms: ATP Binding Cassette Transporter G1; Human ATP Binding Cassette Transporter G1 ELISA Kit; ABCG1 (ATP Binding Cassette Transporter G1); ABCG1 elisa kit

• Reactivity: Human
• Sequence Length: 785

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ABCG1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ABCG1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ABCG1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ABCG1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ABCG1can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 16304311
• NCBI GeneID: 9619
• Molecular Weight: 86,628 Da
• NCBI Official Full Name: ATP-binding cassette transporter G1
• NCBI Official Synonym Full Names: ATP binding cassette subfamily G member 1
• NCBI Official Symbol: ABCG1
• NCBI Official Synonym Symbols: ABC8; WHITE1
• NCBI Protein Information: ATP-binding cassette sub-family G member 1
• UniProt Protein Name: ATP-binding cassette sub-family G member 1
• Protein Family: ABC transporter G family
• UniProt Gene Name: ABCG1
• UniProt Synonym Gene Names: ABC8; WHT1
• UniProt Entry Name: ABCG1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. Six alternative splice variants have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCG1: Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ABC family

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: endosome; external side of plasma membrane; Golgi apparatus; integral to membrane; integral to plasma membrane; intracellular membrane-bound organelle; mitochondrion; nucleoplasm; plasma membrane; recycling endosome

Molecular Function: ADP binding; ATP binding; cholesterol binding; cholesterol transporter activity; phospholipid binding; phospholipid transporter activity; protein heterodimerization activity; protein homodimerization activity; sterol-transporting ATPase activity; toxin transporter activity

Biological Process: amyloid precursor protein catabolic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; intracellular cholesterol transport; lipoprotein metabolic process; phospholipid efflux; phospholipid homeostasis; positive regulation of cholesterol biosynthetic process; regulation of transcription, DNA-dependent; response to lipid; response to organic substance; reverse cholesterol transport; transmembrane transport