Mitochondrial Isocitrate Dehydrogenase (ICDHm) Activity Assay Kit | ICDHm assay kit
Mitochondrial Isocitrate Dehydrogenase (ICDHm) Activity Colorimetric Assay Kit
Synonyms
Mitochondrial Isocitrate Dehydrogenase (ICDHm) Activity; Mitochondrial Isocitrate Dehydrogenase (ICDHm) Activity Colorimetric Assay Kit; ICDHm assay kit
Tag
ICDHm
Features & Benefits
simple, convenient, rapid and colorimetric Mitochondrial Isocitrate Dehydrogenase (ICDHm) activity detection method
compatible to various biological samples, especially animals or plant tissues or cell cultures (adherent or suspension) and fungi.
Provides detailed sample preparation and results calculation methods.
Mitochondrial Isocitrate Dehydrogenase: ICDHm Assay Kit provides a simple, convenient, rapid and colorimetric ICDHm activity detection method, which is compatible to various biological samples, especially animals or plant tissues or cell cultures: adherent or suspension and fungi. The principle is that ICDHM can catalyze NADP + reduction to produce NADPH, NADPH has an absorption peak at 340nm, and ICDHM activity can be calculated by the change of light absorption at 340nm.
compatible to various biological samples, especially animals or plant tissues or cell cultures (adherent or suspension) and fungi.
Provides detailed sample preparation and results calculation methods.
Mitochondrial Isocitrate Dehydrogenase: ICDHm Assay Kit provides a simple, convenient, rapid and colorimetric ICDHm activity detection method, which is compatible to various biological samples, especially animals or plant tissues or cell cultures: adherent or suspension and fungi. The principle is that ICDHM can catalyze NADP + reduction to produce NADPH, NADPH has an absorption peak at 340nm, and ICDHM activity can be calculated by the change of light absorption at 340nm.
Kit Components
Extraction Buffer
ReagentI
Reagent II
Reagent III
Reagent IV
Reagent V
ReagentI
Reagent II
Reagent III
Reagent IV
Reagent V
Usage Notes
deltaA of Test Tube should be between 0.01-0.3, the sample should be diluted further if deltaA of Test Tube >0.3.
Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
Avoid bubbles while mixing or redissolving components.
Change pipette tips frequently to avoid cross contamination between components.
Ensure that all components and equipment are at the proper temperature before starting the experiment.
All samples and reagents should be on ice to avoid denaturation and deactivation.
Don't recommend to test too many samples in one time,because enzyme activity is calculated by the variation of the absorbance value per unit time.
Recommended to calculate the enzyme activity by protein concentration. If you need to calculate by the sample weight, the total enzyme activity is equal to the enzyme activity of the supernatant and pellet which is cytoplasmic extraction.
Please to extract ICDHm from fresh samples in order to ensure the enzyme activity.
Please reduce the tissues weight if the OD values is too high. Or reduce the volume of Extraction Buffer if the OD values is too low.
Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
Avoid bubbles while mixing or redissolving components.
Change pipette tips frequently to avoid cross contamination between components.
Ensure that all components and equipment are at the proper temperature before starting the experiment.
All samples and reagents should be on ice to avoid denaturation and deactivation.
Don't recommend to test too many samples in one time,because enzyme activity is calculated by the variation of the absorbance value per unit time.
Recommended to calculate the enzyme activity by protein concentration. If you need to calculate by the sample weight, the total enzyme activity is equal to the enzyme activity of the supernatant and pellet which is cytoplasmic extraction.
Please to extract ICDHm from fresh samples in order to ensure the enzyme activity.
Please reduce the tissues weight if the OD values is too high. Or reduce the volume of Extraction Buffer if the OD values is too low.
Preparation and Storage
The kit is valid for 6 months. Please refer to the table below Materials supplied and Storage conditions to store all the components.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
Related Product Information for ICDHm assay kit
Description: Mitochondrial Isocitrate Dehydrogenase (ICDHm) Assay Kit provides a simple, convenient, rapid and colorimetric ICDHm activity detection method, which is compatible to various biological samples, especially animals or plant tissues or cell cultures (adherent or suspension) and fungi.
Background: Mitochondrial Isocitrate Dehydrogenase (ICDHM) is widely present in mitochondria of animals, plants, microorganisms, and cultured cells. It can catalyze Isocitrate decarboxylic reaction and produce alpha-Ketoglutarate, and reduce NADP+ to NADPH.
Background: Mitochondrial Isocitrate Dehydrogenase (ICDHM) is widely present in mitochondria of animals, plants, microorganisms, and cultured cells. It can catalyze Isocitrate decarboxylic reaction and produce alpha-Ketoglutarate, and reduce NADP+ to NADPH.
Product Categories/Family for ICDHm assay kit
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Product Notes
The ICDHm (Catalog #AAA9719207) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Mitochondrial Isocitrate Dehydrogenase (ICDHm) Activity, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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