Mouse C-type lectin domain family 12 member A (CLEC12A) ELISA Kit | CLEC12A elisa kit

Mouse C-type lectin domain family 12 member A (CLEC12A) ELISA Kit

Cat. Num. AAA7212626

• Gene Names: CLEC12A; CLL1; MICL; CLL-1; DCAL-2
• Reactivity: Mouse
• Synonyms: C-type lectin domain family 12 member A (CLEC12A); Mouse C-type lectin domain family 12 member A (CLEC12A) ELISA Kit; CLEC12A elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This CLEC12A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CLEC12A. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CLEC12A elisa kit

Principle of the assay: CLEC12A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CLEC12A antibody and an CLEC12A-HRP conjugate. The assay sample and buffer are incubated together with CLEC12A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC12A concentration since CLEC12A from samples and CLEC12A-HRP conjugate compete for the anti-CLEC12A antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC12A from the sample, fewer sites are left to bind CLEC12A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC12A concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CLEC12A elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 308153619
• NCBI GeneID: 160364
• Molecular Weight: 24,449 Da
• NCBI Official Full Name: C-type lectin domain family 12 member A
• NCBI Official Synonym Full Names: C-type lectin domain family 12, member A
• NCBI Official Symbol: CLEC12A
• NCBI Official Synonym Symbols: CLL1; MICL; CLL-1; DCAL-2
• NCBI Protein Information: C-type lectin domain family 12 member A; C-type lectin protein CLL-1; C-type lectin-like molecule-1; dendritic cell-associated lectin 2; myeloid inhibitory C-type lectin-like receptor
• UniProt Protein Name: C-type lectin domain family 12 member A
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CLEC12A
• UniProt Synonym Gene Names: CLL1; DCAL2; MICL; CLL-1; DCAL-2; MICL
• UniProt Entry Name: CL12A_HUMAN

NCBI Description

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signaling, glycoprotein turnover, and roles in inflammation and immune response. The protein encoded by this gene is a negative regulator of granulocyte and monocyte function. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. This gene is closely linked to other CTL/CTLD superfamily members in the natural killer gene complex region on chromosome 12p13. [provided by RefSeq, May 2011]

Uniprot Description

CLEC12A: Cell surface receptor that modulates signaling cascades and mediates tyrosine phosphorylation of target MAP kinases. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: plasma membrane; integral to membrane

Molecular Function: carbohydrate binding