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Mitochondrial complex IV Assay Kit

Mitochondrial complex IV Activity Assay Kit (colorimetric)

Synonyms
Mitochondrial complex IV; Mitochondrial complex IV Activity Assay Kit (colorimetric); Mitochondrial complex IV assay kit
Ordering
For Research Use Only!
Application Notes
Mitochondrial complex IV Activity Detection Kit (colorimetric) is specially developed for the detection of complex I activity. The operation is simple and convenient, and the detection is more sensitive and accurate. Reduced cytochrome C has characteristic light absorption at 550nm. Mitochondrial respiratory chain complex IV could catalyze reduced cytochrome C to oxidized cytochrome C. Therefore, the decreasing rate of light absorption at 550nm can reflect the enzyme activity of mitochondrial respiratory chain complex IV.
Preparation and Storage
The kit is valid for est. 6 months. Please refer to the table below materials supplied and storage conditions to store all the components.
Shipping: Gel pack with blue ice.
Related Product Information for Mitochondrial complex IV assay kit
Principle of the Assay: Reduced cytochrome C has characteristic light absorption at 550nm. Mitochondrial respiratory chain complex IV could catalyze reduced cytochrome C to oxidized cytochrome C. Therefore, the decreasing rate of light absorption at 550nm can reflect the enzyme activity of mitochondrial respiratory chain complex IV. It can be used to determine animal, plant tissue and cell samples.

Background/Introduction: Mitochondrial respiratory chain complex IV, also known as cytochrome C oxidase, is also a common component of the main and branch pathways of the mitochondrial respiratory chain. It is responsible for catalyzing the oxidation of reduced cytochrome C, and ultimately transferring electrons to oxygen to produce water.
Product Categories/Family for Mitochondrial complex IV assay kit

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Product Notes

The Mitochondrial complex IV (Catalog #AAA9719097) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Mitochondrial complex IV Activity Detection Kit (colorimetric) is specially developed for the detection of complex I activity. The operation is simple and convenient, and the detection is more sensitive and accurate. Reduced cytochrome C has characteristic light absorption at 550nm. Mitochondrial respiratory chain complex IV could catalyze reduced cytochrome C to oxidized cytochrome C. Therefore, the decreasing rate of light absorption at 550nm can reflect the enzyme activity of mitochondrial respiratory chain complex IV. Researchers should empirically determine the suitability of the Mitochondrial complex IV for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mitochondrial complex IV, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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