Typical Testing Data/Standard Curve (for reference only)
Human Leucyl/Cystinyl Aminopeptidase (LNPEP) ELISA Kit | LNPEP elisa kit
Human Leucyl/Cystinyl Aminopeptidase (LNPEP) ELISA Kit
Gene Names
LNPEP; CAP; IRAP; PLAP; P-LAP
Reactivity
Human
Synonyms
Leucyl/Cystinyl Aminopeptidase (LNPEP); Human Leucyl/Cystinyl Aminopeptidase (LNPEP) ELISA Kit; CAP; PLAP; OTASE; IRAP; Oxytocinase; Insulin-regulated membrane aminopeptidase; Insulin-responsive aminopeptidase; Placental leucine aminopeptidase; LNPEP elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of lipopolysaccharide. No significant cross-reactivity or interference between lipopolysaccharide and analogues was observed.
Sequence Length
1,006
Samples
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.058ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level lipopolysaccharide were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level lipopolysaccharide were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LNPEP elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of lipopolysaccharide in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to lipopolysaccharide. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to lipopolysaccharide. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain lipopolysaccharide, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of lipopolysaccharide in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to lipopolysaccharide. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to lipopolysaccharide. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain lipopolysaccharide, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of lipopolysaccharide in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
115,062 Da
NCBI Official Full Name
leucyl/cystinyl aminopeptidase
NCBI Official Synonym Full Names
leucyl and cystinyl aminopeptidase
NCBI Official Symbol
LNPEP
NCBI Official Synonym Symbols
CAP; IRAP; PLAP; P-LAP
NCBI Protein Information
leucyl-cystinyl aminopeptidase
UniProt Protein Name
Leucyl-cystinyl aminopeptidase
Protein Family
UniProt Gene Name
LNPEP
UniProt Synonym Gene Names
OTASE; Cystinyl aminopeptidase; IRAP; OTase; P-LAP
NCBI Description
This gene encodes a zinc-dependent aminopeptidase that cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin, dynorphin A and other peptide hormones. The protein can be secreted in maternal serum, reside in intracellular vesicles with the insulin-responsive glucose transporter GLUT4, or form a type II integral membrane glycoprotein. The protein catalyzes the final step in the conversion of angiotensinogen to angiotensin IV (AT4) and is also a receptor for AT4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
Release of an N-terminal amino acid, cleaves before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain.
Research Articles on LNPEP
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Product Notes
The Human LNPEP lnpep (Catalog #AAA2706476) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2706476 ELISA Kit recognizes Human LNPEP. It is sometimes possible for the material contained within the vial of "Leucyl/Cystinyl Aminopeptidase (LNPEP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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