Mitochondrial complex I Assay Kit
Mitochondrial complex I Activity Assay Kit (colorimetric)
Synonyms
Mitochondrial complex I; Mitochondrial complex I Activity Assay Kit (colorimetric); Mitochondrial complex I assay kit
Application Notes
Mitochondrial Complex I Activity Detection Kit (colorimetric) is specially developed for the detection of complex I activity. The operation is simple and convenient, and the detection is more sensitive and accurate. Complex I can catalyze the dehydrogenation of NADH to NAD+. The oxidation rate of NADH can be determined at 340nm to calculate the activity of Complex I.
Preparation and Storage
The kit is valid for est. 6 months. Please refer to the table below materials supplied and storage conditions to store all the components.
Shipping: Gel pack with blue ice.
Shipping: Gel pack with blue ice.
Related Product Information for Mitochondrial complex I assay kit
Principle of the Assay: Complex I can catalyze the dehydrogenation of NADH to NAD+. The oxidation rate of NADH can be determined at 340 nm to calculate the activity of Complex I. It can be used to determine animal, plant tissue and cell samples.
Background/Introduction: Mitochondrial respiratory chain complex I (EC 1.6.5. 3), also known as NADH-CoQ reductase or NADH dehydrogenase, is the largest protein complex in the mitochondrial intima. Complex I is widely found in the mitochondria of animals, plants, microorganisms and cultured cells. This enzyme catalyzes the transfer of a pair of electrons from NADH to CoQ, and at the same time it can reduce O2 to produce O2-, which is the main part of the respiration electron transport chain to produce O2-. The activity of Complex I can not only reflect the status of responsibility electron transfer chain (ETC), but also reflect the production of reactive oxygen species (ROS).
Background/Introduction: Mitochondrial respiratory chain complex I (EC 1.6.5. 3), also known as NADH-CoQ reductase or NADH dehydrogenase, is the largest protein complex in the mitochondrial intima. Complex I is widely found in the mitochondria of animals, plants, microorganisms and cultured cells. This enzyme catalyzes the transfer of a pair of electrons from NADH to CoQ, and at the same time it can reduce O2 to produce O2-, which is the main part of the respiration electron transport chain to produce O2-. The activity of Complex I can not only reflect the status of responsibility electron transfer chain (ETC), but also reflect the production of reactive oxygen species (ROS).
Product Categories/Family for Mitochondrial complex I assay kit
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Product Notes
The Mitochondrial complex I (Catalog #AAA9719094) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Mitochondrial Complex I Activity Detection Kit (colorimetric) is specially developed for the detection of complex I activity. The operation is simple and convenient, and the detection is more sensitive and accurate. Complex I can catalyze the dehydrogenation of NADH to NAD+. The oxidation rate of NADH can be determined at 340nm to calculate the activity of Complex I. Researchers should empirically determine the suitability of the Mitochondrial complex I for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mitochondrial complex I, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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