GYPA / CD235a / Glycophorin A Recombinant Protein | GYPA / CD235a recombinant protein

Recombinant Human GYPA / CD235a / Glycophorin A Protein (His tag)

Cat. Num. AAA2545204

• Gene Names: GYPA; MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C)
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Synonyms: GYPA / CD235a / Glycophorin A; Recombinant Human GYPA / CD235a / Glycophorin A Protein (His tag); CD235a; GPA; GPErik; GPSAT; HGpMiV; HGpMiXI; HGpSta(C); MN; MNS; PAS-2; GYPA / CD235a recombinant protein

• Host: HEK293 Cells
• Purity/Purification: > 95 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.
• Sequence Positions: Met 1-Glu91

• Application Notes: The recombinant human GYPA comprises 83 amino acids and has a predicted molecular mass of 9.4 kDa. The apparent molecular mass of the protein is approximately 35-45 and 20 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Species: Human
• Tag: C-His
• Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method.
• Reconstitution: It is recommended that sterile water (500uL) be added to the vial to prepare a stock solution of 2mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; ; Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

SDS-PAGE

Related Product Information for GYPA / CD235a recombinant protein

Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-Uand Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB.

NCBI and Uniprot Product Information

• NCBI GI #: 815891063
• NCBI GeneID: 2993
• NCBI Accession #: NP_001295119.1
• NCBI GenBank Nucleotide #: NM_001308190.1
• UniProt Accession #: P02724; Q9BS51; A8K3E6; B8Q182; B8Q185
• Molecular Weight: Mol_Mass: 9.4 kDa; AP_Mol_Mass: 35-45 & 20 kDa
• NCBI Official Full Name: glycophorin-A isoform 3
• NCBI Official Synonym Full Names: glycophorin A (MNS blood group)
• NCBI Official Symbol: GYPA
• NCBI Official Synonym Symbols: MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C)
• NCBI Protein Information: glycophorin-A
• UniProt Protein Name: Glycophorin-A
• UniProt Gene Name: GYPA
• UniProt Synonym Gene Names: GPA

NCBI Description

Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]

Uniprot Description

Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).