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Western Blot (WB) (Western BlotPositive WB detected in: Rat brain tissueAll lanes: GHITM antibody at 2.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 38 KDaObserved band size: 38 KDa)

Rabbit anti-Human, mouse GHITM Polyclonal Antibody | anti-GHITM antibody

GHITM Antibody

Gene Names
GHITM; DERP2; MICS1; My021; PTD010; TMBIM5; HSPC282
Reactivity
Human, mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purified
Synonyms
GHITM; Polyclonal Antibody; GHITM Antibody; Growth hormone-inducible transmembrane protein; DERP2; MICS1; TMBIM5; My021; UNQ244/PRO281; Dermal papilla-derived protein 2; Mitochondrial morphology and cristae structure 1; Transmembrane BAX inhibitor motif-containing protein 5; anti-GHITM antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Liquid
Sequence Length
345
Applicable Applications for anti-GHITM antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Recommended dilution: WB:1:500-2000, IHC:1:20-1:200
Immunogen
Recombinant human Growth hormone-inducible transmembrane protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-138015-R
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western BlotPositive WB detected in: Rat brain tissueAll lanes: GHITM antibody at 2.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 38 KDaObserved band size: 38 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: Rat brain tissueAll lanes: GHITM antibody at 2.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 38 KDaObserved band size: 38 KDa)

Immunohistochemistry (IHC)

(IHC image of MBS7000658 diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC) (IHC image of MBS7000658 diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC)

(IHC image of MBS7000658 diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC) (IHC image of MBS7000658 diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunofluorescence (IF)

(Immunofluorescent analysis of HepG2 cells using MBS7000658 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunofluorescence (IF) (Immunofluorescent analysis of HepG2 cells using MBS7000658 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-GHITM antibody
Required for the mitochondrial tubular network and cristae organization. Involved in apoptotic release of cytochrome c.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,205 Da
NCBI Official Full Name
growth hormone-inducible transmembrane protein
NCBI Official Synonym Full Names
growth hormone inducible transmembrane protein
NCBI Official Symbol
GHITM
NCBI Official Synonym Symbols
DERP2; MICS1; My021; PTD010; TMBIM5; HSPC282
NCBI Protein Information
growth hormone-inducible transmembrane protein
UniProt Protein Name
Growth hormone-inducible transmembrane protein
UniProt Gene Name
GHITM
UniProt Synonym Gene Names
DERP2; MICS1; TMBIM5; MICS1
UniProt Entry Name
GHITM_HUMAN

Uniprot Description

GHITM: Required for the mitochondrial tubular network and cristae organization. Involved in apoptotic release of cytochrome c. Belongs to the BI1 family.

Protein type: Membrane protein, integral; Mitochondrial; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 10q23.1

Cellular Component: integral to membrane; mitochondrial inner membrane

Molecular Function: protein binding

Biological Process: apoptosis

Research Articles on GHITM

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Product Notes

The GHITM ghitm (Catalog #AAA7000658) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GHITM Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GHITM can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the GHITM ghitm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GHITM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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