Diamine Oxidase (DAO) Assay Kit | DAO assay kit
Diamine Oxidase (DAO) Activity Colorimetric Assay Kit
Synonyms
Diamine Oxidase (DAO); Diamine Oxidase (DAO) Activity Colorimetric Assay Kit; Amine oxidase; copper-containing 1; AOC1; Histaminase; DAO assay kit
Application Notes
DAO catalyzed resin amine to produce aldehyde and hydrogen peroxide, and add excessive horseradish peroxidase exogenous to catalyze hydrogen peroxide to oxidize o-anisamine to produce colored substances which has a characteristic absorption peak at 460nm. DAO activity is calculated by measuring the absorbance increase rate of this wavelength. It can test samples of tissue, cells, bacteria, plants and serum (plasma).
Preparation and Storage
Store at 4 degree C and keep from light immediately upon receipt. Kit has a storage time of est. 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping: Gel pack with blue ice.
Shipping: Gel pack with blue ice.
Related Product Information for DAO assay kit
Principle of the Assay: Diamine oxidase (DAO) catalyzes cadaverine to produce aldehyde and hydrogen peroxide. Excessive addition of horseradish peroxidase catalyzes the oxidation of o-dianisidine by hydrogen peroxide to produce colored substances with a characteristic absorption peak at 460 nm, This kit can detect samples of animal and plant tissues, cells, bacteria, serum (plasma), etc.
Background/Introduction: DAO(EC1.4.3.6) is widely present in animals (intestinal mucosa, lung, liver, kidney), plants, microorganisms. It catalyzes polyamine and oxidized to aldehydes, Its activity is closely related to nucleic acid and protein synthesis and can reflect the integrity of the intestinal mechanical barrier and the degree of damage.
Background/Introduction: DAO(EC1.4.3.6) is widely present in animals (intestinal mucosa, lung, liver, kidney), plants, microorganisms. It catalyzes polyamine and oxidized to aldehydes, Its activity is closely related to nucleic acid and protein synthesis and can reflect the integrity of the intestinal mechanical barrier and the degree of damage.
Product Categories/Family for DAO assay kit
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Product Notes
The DAO (Catalog #AAA9719025) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. DAO catalyzed resin amine to produce aldehyde and hydrogen peroxide, and add excessive horseradish peroxidase exogenous to catalyze hydrogen peroxide to oxidize o-anisamine to produce colored substances which has a characteristic absorption peak at 460nm. DAO activity is calculated by measuring the absorbance increase rate of this wavelength. It can test samples of tissue, cells, bacteria, plants and serum (plasma). Researchers should empirically determine the suitability of the DAO for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Diamine Oxidase (DAO), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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