Mouse Diacylglycerol Kinase alpha (DGKA) ELISA Kit | DGKA elisa kit

Mouse Diacylglycerol Kinase alpha (DGKA) ELISA Kit

Cat. Num. AAA9717909

• Gene Names: Dgka; 80kDa; Dagk1; AW146112
• Reactivity: Mouse
• Synonyms: Diacylglycerol Kinase alpha (DGKA); Mouse Diacylglycerol Kinase alpha (DGKA) ELISA Kit; DGKA; DAGK; DAGK1; DGK-alpha; MGC12821; MGC42356; diacylglycerol kinase alpha; DGKA elisa kit

• Reactivity: Mouse
• Specificity: Mouse Diacylglycerol kinase alpha (DGKA) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DGKA. No significant cross-reactivity or interference between Mouse DGKA and analogues was observed.
• Sequence Length: 730

• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Diacylglycerol kinase alpha (DGKA) Standard Curve.)

Related Product Information for DGKA elisa kit

Principle of the Assay: miRNAs real-time Quantitation assay of miRNAs includes two steps, stem-loop RT and real-time PCR. Stem-loop RT primers bind to at the 3' portion of miRNA molecules and are reverse transcribed with reverse transcriptase. Then, the RT product is quantified using real-time PCR that includes miRNA-specific forward primer, reverse primer and SYBR Green dye!!Background/Introduction: The miRNAs RT-PCR Quantitation Kit is a sensitive and specific method using real-time PCR for the detection and quantification of miRNAs (miRNA) from total RNA samples. miRNAs are small, single-stranded, ~19-23 nt RNA molecules encoded in the genomes of plants, animals, and viruses. Mature miRNAs enter the RNA-induced silencing complex (RISC) and guide the RISC to induce translational repression or endonucleolytic cleavage of specific target mRNAs. Unlike commonly used methods for detection of miRNAs, the miRNAs qPCR Quantitation Kit is more rapid and sensitive. This kit contains miRNA hsa-mir-130b and cel-mir-39-3p high specific RT and PCR primer set, with SYBR Green dye included. The stem-loop like miRNAs RT primer and the miRNAs high specific primer set ensure the RT and PCR reaction would not be interfered by the miRNAs precursors. The reaction template could be total RNA or cell lysates. The system enables highly sensitive detection from as few as 10 copies of a target miRNAs, with a broad dynamic range that supports accurate quantification of high-copy mRNA from up to 1 ug of total RNA. High specificity ensurenced by stemloop RT primer and high specific miRNAs primer set; even highly homogized miRNAs can be accurately dicriminated. Higher dynamic range and sensitivity of miRNAs quantification than conventional method (such as Northern blot & micro-array) with broad dynamic range of at least seven orders of magnitude, and in this ready to use formulation can quantify as few as 7 copies of a miRNA target in as little as 0.1 pg of total RNA. Less sample requirement Total RNA, cell lysate or purified samll RNA can work as the qPCR Quantitation Kit's template, even the genomic DNA contamination would not interfere in miRNAs quantification. miRNA synthetic standard can work as a standard curve when you need to know the absolute number of a miRNA in one cell or work as positive control when you need to know the relative expression ratio between miRNA and cel-mir-39-3p or 5S rRNA.

NCBI and Uniprot Product Information

• NCBI GI #: 31560474
• NCBI GeneID: 13139
• NCBI Accession #: NP_058091.2
• NCBI GenBank Nucleotide #: NP_058091.2
• UniProt Accession #: O88673
• NCBI Official Full Name: diacylglycerol kinase alpha
• NCBI Official Synonym Full Names: diacylglycerol kinase, alpha
• NCBI Official Symbol: Dgka
• NCBI Official Synonym Symbols: 80kDa; Dagk1; AW146112
• NCBI Protein Information: diacylglycerol kinase alpha
• UniProt Protein Name: Diacylglycerol kinase alpha
• Protein Family: Diacylglycerol kinase
• UniProt Gene Name: Dgka
• UniProt Synonym Gene Names: Dagk1; DAG kinase alpha; DGK-alpha
• UniProt Entry Name: DGKA_MOUSE