Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Melanocortin receptor 5 (MC5R) Standard Curve.)
Mouse Melanocortin Receptor 5 (MC5R) ELISA Kit | MC5R elisa kit
Mouse Melanocortin Receptor 5 (MC5R) ELISA Kit
Reactivity
Mouse
Synonyms
Melanocortin Receptor 5 (MC5R); Mouse Melanocortin Receptor 5 (MC5R) ELISA Kit; MC5R; Melanocortin receptor 5; MC5R elisa kit
Reactivity
Mouse
Specificity
Mouse Melanocortin receptor 5 (MC5R) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MC5R. No significant cross-reactivity or interference between Mouse MC5R and analogues was observed.
Sequence Length
325
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Melanocortin receptor 5 (MC5R) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Melanocortin receptor 5 (MC5R) Standard Curve.)
Related Product Information for MC5R elisa kit
Description: This Mouse Melanocortin receptor 5 (MC5R) ELISA Kit employs a two-site sandwich ELISA to quantitate MC5R in samples. An antibody specific for MC5R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MC5R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MC5R is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MC5R bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Melanocortin receptor 5 is located on the chromosome 18 in the human genome. when the melanocortin 5 receptor was disrupted in transgenic mice, it induced disruption of their exocrine glands and resulted in decreased production of sebum. Melanocortin-1 receptor (MC1R) is found in melanocytes and appears to be the alpha-melanocyte stimulating hormone (MSH) receptor. MC2R is localized to adrenocortical cells and is thus thought to be the ACTH receptor. MC3R and MC4R are localized primarily to sites in the brain. Each receptor has a unique pattern of affinity for the various melanocortin ligands. Gantz et al. (1994) reported the molecular cloning of a novel gene encoding a fifth member of the MCR family in the mouse. Chowdhary et al. (1995) mapped the human MC5R gene to 18p11.2 by fluorescence in situ hybridization.
Background: Melanocortin receptor 5 is located on the chromosome 18 in the human genome. when the melanocortin 5 receptor was disrupted in transgenic mice, it induced disruption of their exocrine glands and resulted in decreased production of sebum. Melanocortin-1 receptor (MC1R) is found in melanocytes and appears to be the alpha-melanocyte stimulating hormone (MSH) receptor. MC2R is localized to adrenocortical cells and is thus thought to be the ACTH receptor. MC3R and MC4R are localized primarily to sites in the brain. Each receptor has a unique pattern of affinity for the various melanocortin ligands. Gantz et al. (1994) reported the molecular cloning of a novel gene encoding a fifth member of the MCR family in the mouse. Chowdhary et al. (1995) mapped the human MC5R gene to 18p11.2 by fluorescence in situ hybridization.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Melanocortin receptor 5
NCBI Official Synonym Full Names
melanocortin 5 receptor
NCBI Official Symbol
Mc5r
NCBI Protein Information
melanocortin receptor 5
UniProt Protein Name
Melanocortin receptor 5
Protein Family
UniProt Gene Name
Mc5r
UniProt Synonym Gene Names
MC5-R
UniProt Entry Name
MC5R_MOUSE
Uniprot Description
MC5R: Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. This receptor is a possible mediator of the immunomodulation properties of melanocortins. Belongs to the G-protein coupled receptor 1 family.
Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR
Cellular Component: membrane; plasma membrane; integral to membrane
Molecular Function: G-protein coupled receptor activity; signal transducer activity; hormone binding; melanocortin receptor activity
Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of cAMP biosynthetic process; signal transduction
Research Articles on MC5R
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Product Notes
The Mouse MC5R mc5r (Catalog #AAA9717708) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717708 ELISA Kit recognizes Mouse MC5R. It is sometimes possible for the material contained within the vial of "Melanocortin Receptor 5 (MC5R), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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