Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Melanocortin receptor 4 (MC4R) Standard Curve.)
Mouse Melanocortin Receptor 4 (MC4R) ELISA Kit | MC4R elisa kit
Mouse Melanocortin Receptor 4 (MC4R) ELISA Kit
Gene Names
Mc4r; Pkcp; Mc4-r
Reactivity
Mouse
Synonyms
Melanocortin Receptor 4 (MC4R); Mouse Melanocortin Receptor 4 (MC4R) ELISA Kit; MC4R; MGC126851; MGC138197; MC4R elisa kit
Reactivity
Mouse
Specificity
Mouse Melanocortin receptor 4 (MC4R) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MC4R. No significant cross-reactivity or interference between Mouse MC4R and analogues was observed.
Sequence Length
332
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Melanocortin receptor 4 (MC4R) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Melanocortin receptor 4 (MC4R) Standard Curve.)
Related Product Information for MC4R elisa kit
Description: This Mouse Melanocortin receptor 4 (MC4R) ELISA Kit employs a two-site sandwich ELISA to quantitate MC4R in samples. An antibody specific for MC4R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MC4R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MC4R is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MC4R bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Melanocortin receptor 4 is a protein that in humans is encoded by the MC4R gene. It encodes the MC4 protein, a G-protein coupled receptor that binds alpha-melanocyte stimulating hormone (alpha-MSH). In murine models MC4 receptors have been found to be involved in feeding behaviour, the regulation of metabolism, sexual behaviour, and male erectile function. In 1998, it was reported that MC4R mutations were associated with inherited human obesity. They were found in heterozygotes, suggesting an autosomal dominant inheritance pattern. However, based on other research and observations, these mutations seem to have an incomplete penetrance and some degree of codominance. It has a prevalence of 1-2.5% in people with BMIs of greater than 30, making it the most commonly known genetic defect predisposing people to obesity.
Background: Melanocortin receptor 4 is a protein that in humans is encoded by the MC4R gene. It encodes the MC4 protein, a G-protein coupled receptor that binds alpha-melanocyte stimulating hormone (alpha-MSH). In murine models MC4 receptors have been found to be involved in feeding behaviour, the regulation of metabolism, sexual behaviour, and male erectile function. In 1998, it was reported that MC4R mutations were associated with inherited human obesity. They were found in heterozygotes, suggesting an autosomal dominant inheritance pattern. However, based on other research and observations, these mutations seem to have an incomplete penetrance and some degree of codominance. It has a prevalence of 1-2.5% in people with BMIs of greater than 30, making it the most commonly known genetic defect predisposing people to obesity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
melanocortin receptor 4
NCBI Official Synonym Full Names
melanocortin 4 receptor
NCBI Official Symbol
Mc4r
NCBI Official Synonym Symbols
Pkcp; Mc4-r
NCBI Protein Information
melanocortin receptor 4
UniProt Protein Name
Melanocortin receptor 4
Protein Family
UniProt Gene Name
Mc4r
UniProt Synonym Gene Names
MC4-R
UniProt Entry Name
MC4R_MOUSE
NCBI Description
This gene encodes a member of the melanocortin receptor family. Melanocortin receptors are transmembrane G-protein coupled receptors, which respond to small peptide hormones and exhibit diverse functions and tissue type localization. As part of the central nervous melanocortin system, the encoded protein is competitively bound by either melanocyte stimulating hormone or agouti-related protein to regulate energy homeostasis. Disruption of this gene promotes hyperphagia and obesity, and is associated with increased cholesterol levels and insulin resistance. [provided by RefSeq, Dec 2012]
Uniprot Description
Receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. Plays a central role in energy homeostasis and somatic growth. This receptor is mediated by G proteins that stimulate adenylate cyclase (cAMP).
Research Articles on MC4R
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Product Notes
The Mouse MC4R mc4r (Catalog #AAA9717709) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717709 ELISA Kit recognizes Mouse MC4R. It is sometimes possible for the material contained within the vial of "Melanocortin Receptor 4 (MC4R), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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