Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Probable E3 ubiquitin-protein ligase MID2 (MID2) Standard Curve.)
Mouse Probable E3 Ubiquitin-Protein Ligase MID2 (MID2) ELISA Kit | MID2 elisa kit
Mouse Probable E3 Ubiquitin-Protein Ligase MID2 (MID2) ELISA Kit
Gene Names
Mid2; FXY2; Trim1
Reactivity
Mouse
Synonyms
Probable E3 Ubiquitin-Protein Ligase MID2 (MID2); Mouse Probable E3 Ubiquitin-Protein Ligase MID2 (MID2) ELISA Kit; MID2; RP6-191P20.2; FLJ37715; FLJ41813; FXY2; RNF60; TRIM1; midin 2; tripartite motif protein 1; MID2 elisa kit
Reactivity
Mouse
Specificity
Mouse Probable E3 ubiquitin-protein ligase MID2 (MID2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MID2. No significant cross-reactivity or interference between Mouse MID2 and analogues was observed.
Sequence Length
685
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Probable E3 ubiquitin-protein ligase MID2 (MID2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Probable E3 ubiquitin-protein ligase MID2 (MID2) Standard Curve.)
Related Product Information for MID2 elisa kit
Description: This Mouse Probable E3 ubiquitin-protein ligase MID2 (MID2) ELISA Kit employs a two-site sandwich ELISA to quantitate MID2 in samples. An antibody specific for MID2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MID2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MID2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MID2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The protein encoded by MID2 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of MID2 results in two transcript variants encoding different isoforms.
Background: The protein encoded by MID2 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of MID2 results in two transcript variants encoding different isoforms.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
probable E3 ubiquitin-protein ligase MID2 isoform 3
NCBI Official Synonym Full Names
midline 2
NCBI Official Symbol
Mid2
NCBI Official Synonym Symbols
FXY2; Trim1
NCBI Protein Information
probable E3 ubiquitin-protein ligase MID2
UniProt Protein Name
Probable E3 ubiquitin-protein ligase MID2
Protein Family
UniProt Gene Name
Mid2
UniProt Synonym Gene Names
Fxy2; Trim1
UniProt Entry Name
TRIM1_MOUSE
Research Articles on MID2
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Product Notes
The Mouse MID2 mid2 (Catalog #AAA9717637) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717637 ELISA Kit recognizes Mouse MID2. It is sometimes possible for the material contained within the vial of "Probable E3 Ubiquitin-Protein Ligase MID2 (MID2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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