Mouse Polymeric Immunoglobulin Receptor (PIGR) ELISA Kit | PIGR elisa kit

Mouse Polymeric Immunoglobulin Receptor (PIGR) ELISA Kit

Cat. Num. AAA9717363

• Reactivity: Mouse
• Synonyms: Polymeric Immunoglobulin Receptor (PIGR); Mouse Polymeric Immunoglobulin Receptor (PIGR) ELISA Kit; PIGR; FLJ22667; MGC125361; MGC125362; hepatocellular carcinoma associated protein TB6; PIGR elisa kit

• Reactivity: Mouse
• Specificity: Mouse Polymeric immunoglobulin receptor (PIGR) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PIGR. No significant cross-reactivity or interference between Mouse PIGR and analogues was observed.
• Sequence Length: 771

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Polymeric immunoglobulin receptor (PIGR) Standard Curve.)

Related Product Information for PIGR elisa kit

Description: This Mouse Polymeric immunoglobulin receptor (PIGR) ELISA Kit employs a two-site sandwich ELISA to quantitate PIGR in samples. An antibody specific for PIGR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIGR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIGR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIGR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Polymeric immunoglobulin receptor is a Fc receptor which facilitates the secretion of IgA and IgM. The poly-Ig receptor is expressed on several glandular epithelia including those of liver and breast. It mediates transcellular transport of polymeric immunoglobulin molecules. It is a member of the immunoglobulin superfamily (Hood et al., 1985). The receptor has 5 units with homology to the variable (V) units of immunoglobulins and a transmembrane region, which also has some homology to certain immunoglobulin variable regions.

NCBI and Uniprot Product Information

• NCBI GI #: 31981570
• NCBI GeneID: 18703
• NCBI Accession #: NP_035212.2
• NCBI GenBank Nucleotide #: NP_035212.2
• UniProt Accession #: O70570
• NCBI Official Full Name: polymeric immunoglobulin receptor
• NCBI Official Synonym Full Names: polymeric immunoglobulin receptor
• NCBI Official Symbol: Pigr
• NCBI Protein Information: polymeric immunoglobulin receptor
• UniProt Protein Name: Polymeric immunoglobulin receptor
• Protein Family: Polymeric immunoglobulin receptor
• UniProt Gene Name: Pigr
• UniProt Synonym Gene Names: PIgR; Poly-Ig receptor

Uniprot Description

This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment ().