PIGR active protein

PIGR Protein, Human, Recombinant (365 Ser/Gly, His Tag)

Cat. Num. AAA8120015

• Purity: >97% as determined by SDS-PAGE
• Synonyms: PIGR; PIGR Protein; Human; Recombinant (365 Ser/Gly; His Tag); Human PIGR Protein (365 Ser/Gly; FLJ22667 Protein; MGC125361 Protein; MGC125362 Protein; polymeric immunoglobulin receptor; PIGR active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg638, 365Ser/Gly

• Species: Human
• Activity: Measured by its binding ability in a functional ELISA.; 1. Immobilized rhhuman IgM at 2ug/ml (100uL/well) can bind biotinylated PIGR with a linear range of 0.94-15ng/mL.; 2. When human human IgM is immobilized at 2ug/ml (100uL/well), PIGR inhibits 50% binding of biotinylated PIGR (0.062ug/ml) at the concentration range of 0.03-20ug/ml.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Lys 19
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Arg 638, 365 Ser/Gly) of human PIGR (NP_002635.2) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PIGR active protein

Background: Polymeric immunoglobulin receptor, also known as PIGR, is a member of the immunoglobulin superfamily and a Fc receptor. The ectodomain of this receptor consists of five units with homology to the variable units of immunoglobulins and a transmembrane region, which also has some homology to certain immunoglobulin variable regions. PIGR is expressed on several glandular epithelia including those of liver and breast. The deduced amino-acid sequence has a length of 764 residues and shows an overall similarity of 56% and 64% with the rabbit and rat counterpart. PIGR mediates transcellular transport of polymeric immunoglobulin molecules, and thus facilitates the secretion of IgA and IgM. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment of PIGR.

References

Coyne, R. S. et al., 1995, J. Biol. Chem. 269 (50) :31620-31625. Kaetzel,C.S., 2001, Curr Biol. 11(1): R35-38.

NCBI and Uniprot Product Information

• NCBI GI #: 31377806
• NCBI GeneID: 5284
• NCBI Accession #: NP_002635.2
• NCBI GenBank Nucleotide #: NM_002644.3
• UniProt Accession #: P01833; Q68D81; Q8IZY7
• Molecular Weight: 83,284 Da
• NCBI Official Full Name: polymeric immunoglobulin receptor
• NCBI Official Synonym Full Names: polymeric immunoglobulin receptor
• NCBI Official Symbol: PIGR
• NCBI Protein Information: polymeric immunoglobulin receptor
• UniProt Protein Name: Polymeric immunoglobulin receptor
• Protein Family: Polymeric immunoglobulin receptor
• UniProt Gene Name: PIGR
• UniProt Synonym Gene Names: PIgR; Poly-Ig receptor

NCBI Description

This gene is a member of the immunoglobulin superfamily. The encoded poly-Ig receptor binds polymeric immunoglobulin molecules at the basolateral surface of epithelial cells; the complex is then transported across the cell to be secreted at the apical surface. A significant association was found between immunoglobulin A nephropathy and several SNPs in this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.