PIGR recombinant protein

Recombinant Human PIGR Protein (365 Ser/Gly, His tag)

Cat. Num. AAA2546496

• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: PIGR; Recombinant Human PIGR Protein (365 Ser/Gly; His tag); FLJ22667; MGC125361; MGC125362; PIGR recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 764

• Application Notes: The soluble form of recombinant human PIGR consists of 630 amino acids after removal of the signal peptide and has a predicted molecular mass of 69 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 80-90 kDa due to glycosylation.
• Predicted N Terminal: Lys 19
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for PIGR recombinant protein

Background: Polymeric immunoglobulin receptor, also known as PIGR, is a member of the immunoglobulin superfamily and a Fc receptor. The ectodomain of this receptor consists of five units with homology to the variable units of immunoglobulins and a transmembrane region, which also has some homology to certain immunoglobulin variable regions. PIGR is expressed on several glandular epithelia including those of liver and breast. The deduced amino-acid sequence has a length of 764 residues and shows an overall similarity of 56% and 64% with the rabbit and rat counterpart. PIGR mediates transcellular transport of polymeric immunoglobulin molecules, and thus facilitates the secretion of IgA and IgM. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment of PIGR.

Description: A DNA sequence encoding the extracellular domain (Met 1-Arg 638, 365 Ser/Gly) of human PIGR (NP_002635.2) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 31377806
• NCBI GeneID: 5284
• NCBI Accession #: NP_002635.2
• NCBI GenBank Nucleotide #: NM_002644.3
• UniProt Accession #: P01833; Q68D81; Q8IZY7
• Molecular Weight: 83,284 Da
• NCBI Official Full Name: polymeric immunoglobulin receptor
• NCBI Official Synonym Full Names: polymeric immunoglobulin receptor
• NCBI Official Symbol: PIGR
• NCBI Protein Information: polymeric immunoglobulin receptor
• UniProt Protein Name: Polymeric immunoglobulin receptor
• Protein Family: Polymeric immunoglobulin receptor
• UniProt Gene Name: PIGR
• UniProt Synonym Gene Names: PIgR; Poly-Ig receptor

NCBI Description

This gene is a member of the immunoglobulin superfamily. The encoded poly-Ig receptor binds polymeric immunoglobulin molecules at the basolateral surface of epithelial cells; the complex is then transported across the cell to be secreted at the apical surface. A significant association was found between immunoglobulin A nephropathy and several SNPs in this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

PIGR: This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.

Protein type: Immunoglobulin superfamily; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: polymeric immunoglobulin receptor activity

Biological Process: detection of chemical stimulus involved in sensory perception of bitter taste; epidermal growth factor receptor signaling pathway; immunoglobulin transcytosis in epithelial cells mediated by polymeric immunoglobulin receptor; receptor clustering; retinal homeostasis

Disease: Iga Nephropathy, Susceptibility To, 1