Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1) Standard Curve.)
Mouse Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase 1 (PLOD1) ELISA Kit | PLOD1 elisa kit
Mouse Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase 1 (PLOD1) ELISA Kit
Gene Names
Plod1; Lh1; Plod; AI854890; AV007929; 2410042F05Rik
Reactivity
Mouse
Synonyms
Procollagen-Lysine; 2-Oxoglutarate 5-Dioxygenase 1 (PLOD1); Mouse Procollagen-Lysine; 2-Oxoglutarate 5-Dioxygenase 1 (PLOD1) ELISA Kit; PLOD1; RP5-1077B9.2; FLJ42041; LH; LH1; LLH; PLOD; lysine hydroxylase; lysyl hydroxylase 1; procollagen-lysine; 2-oxoglutarate 5-dioxygenase; PLOD1 elisa kit
Reactivity
Mouse
Specificity
Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PLOD1. No significant cross-reactivity or interference between Mouse PLOD1 and analogues was observed.
Sequence Length
728
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1) Standard Curve.)
Related Product Information for PLOD1 elisa kit
Description: This Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1) ELISA Kit employs a two-site sandwich ELISA to quantitate PLOD1 in samples. An antibody specific for PLOD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLOD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLOD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLOD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PLOD1 encode a polypeptide of 709 amino acid residues and a signal peptide of 18 amino acids. The human coding sequence shows 72% identity to the chick sequences at the nucleotide level and 76% identity at the amino acid level. The C-terminal region is especially well conserved; residues 588 to 727 show 94% identity between the 2 species and residues 639 to 715 show 99% identity. The gene for lysyl hydroxylase, symbolized PLOD (for procollagen-lysine, 2-oxoglutarate 5-dioxygenase), was mapped to chromosome 1 by Southern blot analysis of human/mouse somatic cell hybrids, to the 1pter-1p34 region by use of cell hybrids that contained various rearrangements of human chromosome 1, and to 1p36.3-p36.2 by in situ hybridization.
Background: PLOD1 encode a polypeptide of 709 amino acid residues and a signal peptide of 18 amino acids. The human coding sequence shows 72% identity to the chick sequences at the nucleotide level and 76% identity at the amino acid level. The C-terminal region is especially well conserved; residues 588 to 727 show 94% identity between the 2 species and residues 639 to 715 show 99% identity. The gene for lysyl hydroxylase, symbolized PLOD (for procollagen-lysine, 2-oxoglutarate 5-dioxygenase), was mapped to chromosome 1 by Southern blot analysis of human/mouse somatic cell hybrids, to the 1pter-1p34 region by use of cell hybrids that contained various rearrangements of human chromosome 1, and to 1p36.3-p36.2 by in situ hybridization.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
NCBI Official Synonym Full Names
procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1
NCBI Official Symbol
Plod1
NCBI Official Synonym Symbols
Lh1; Plod; AI854890; AV007929; 2410042F05Rik
NCBI Protein Information
procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
UniProt Protein Name
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
Protein Family
UniProt Gene Name
Plod1
UniProt Synonym Gene Names
Plod; LH1
UniProt Entry Name
PLOD1_MOUSE
Research Articles on PLOD1
Similar Products
Product Notes
The Mouse PLOD1 plod1 (Catalog #AAA9717335) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717335 ELISA Kit recognizes Mouse PLOD1. It is sometimes possible for the material contained within the vial of "Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase 1 (PLOD1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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