Typical Testing Data/Standard Curve (for reference only)
Guinea Pig Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (PLOD1) ELISA Kit | PLOD1 elisa kit
Guinea pig Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (PLOD1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: PLOD1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PLOD1 antibody and an PLOD1-HRP conjugate. The assay sample and buffer are incubated together with PLOD1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PLOD1 concentration since PLOD1 from samples and PLOD1-HRP conjugate compete for the anti-PLOD1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PLOD1 from the sample, fewer sites are left to bind PLOD1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLOD1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. [provided by RefSeq, Jul 2008]
Uniprot Description
PLOD1: Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. Defects in PLOD1 are the cause of Ehlers-Danlos syndrome type 6 (EDS6). EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS6 is characterized by the presence of ocular complications, particularly retinal detachment. Defects in PLOD1 are the cause of Nevo syndrome (NEVOS). This is a rare, autosomal recessive disorder characterized by increased perinatal length, kyphosis, muscular hypotonia, and joint laxity. Nevo syndrome and EDS-VI have similar clinical phenotypes. Some authors consider that both syndromes are the same clinical entity.
Protein type: Oxidoreductase; Amino Acid Metabolism - lysine degradation; EC 1.14.11.4
Chromosomal Location of Human Ortholog: 1p36.22
Cellular Component: endoplasmic reticulum membrane; rough endoplasmic reticulum membrane
Molecular Function: protein homodimerization activity; L-ascorbic acid binding; procollagen-lysine 5-dioxygenase activity; iron ion binding
Biological Process: hydroxylysine biosynthetic process; extracellular matrix organization and biogenesis; epidermis development; response to hypoxia; protein modification process
Disease: Ehlers-danlos Syndrome, Type Vi
Research Articles on PLOD1
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Product Notes
The Guinea Pig PLOD1 plod1 (Catalog #AAA7223151) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7223151 ELISA Kit recognizes Guinea Pig PLOD1. It is sometimes possible for the material contained within the vial of "Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (PLOD1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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