Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prokineticin receptor 1 (PROKR1) Standard Curve.)
Mouse Prokineticin Receptor 1 (PROKR1) ELISA Kit | PROKR1 elisa kit
Mouse Prokineticin Receptor 1 (PROKR1) ELISA Kit
Gene Names
Prokr1; Pkr1; Gpr73; Pk-r1; EG-VEGFR1
Reactivity
Mouse
Synonyms
Prokineticin Receptor 1 (PROKR1); Mouse Prokineticin Receptor 1 (PROKR1) ELISA Kit; PROKR1; GPR73; GPR73a; PKR1; ZAQ; G protein-coupled receptor 73; G protein-coupled receptor ZAQ; OTTHUMP00000159967; PROKR1 elisa kit
Reactivity
Mouse
Specificity
Mouse Prokineticin receptor 1 (PROKR1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PROKR1. No significant cross-reactivity or interference between Mouse PROKR1 and analogues was observed.
Sequence Length
393
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prokineticin receptor 1 (PROKR1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prokineticin receptor 1 (PROKR1) Standard Curve.)
Related Product Information for PROKR1 elisa kit
Description: This Mouse Prokineticin receptor 1 (PROKR1) ELISA Kit employs a two-site sandwich ELISA to quantitate PROKR1 in samples. An antibody specific for PROKR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PROKR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROKR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROKR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The prokineticin receptor is a G-protein coupled receptor which binds the peptide hormone prokineticin. These receptors mediate gastrointestinal smooth muscle contraction and angiogenesis. There are two variants each encoded by a different gene. Prokineticin receptor 1 (also known as PKR1, or GPR73A), is encoded by the PROKR1 gene. GPR73A is a receptor for prokineticin 1. It is a member of the GPCR family and is coupled to the G(q) subclass of heteromeric G proteins. Activation of the receptor leads to mobilization of calcium, stimulation of phosphoinositide turnover and activation of p44/p42 mitogen-activated protein kinase. GPR73A expression has been reported in adrenal, peripheral blood leukocytes, brain, colon, pancreas, prostate, rectum, salivary gland, small intestine, spleen, stomach, testis, and thyroid.
Background: The prokineticin receptor is a G-protein coupled receptor which binds the peptide hormone prokineticin. These receptors mediate gastrointestinal smooth muscle contraction and angiogenesis. There are two variants each encoded by a different gene. Prokineticin receptor 1 (also known as PKR1, or GPR73A), is encoded by the PROKR1 gene. GPR73A is a receptor for prokineticin 1. It is a member of the GPCR family and is coupled to the G(q) subclass of heteromeric G proteins. Activation of the receptor leads to mobilization of calcium, stimulation of phosphoinositide turnover and activation of p44/p42 mitogen-activated protein kinase. GPR73A expression has been reported in adrenal, peripheral blood leukocytes, brain, colon, pancreas, prostate, rectum, salivary gland, small intestine, spleen, stomach, testis, and thyroid.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
prokineticin receptor 1
NCBI Official Synonym Full Names
prokineticin receptor 1
NCBI Official Symbol
Prokr1
NCBI Official Synonym Symbols
Pkr1; Gpr73; Pk-r1; EG-VEGFR1
NCBI Protein Information
prokineticin receptor 1
UniProt Protein Name
Prokineticin receptor 1
Protein Family
UniProt Gene Name
Prokr1
UniProt Synonym Gene Names
Gpr73; Pkr1; PK-R1
Uniprot Description
Receptor for prokineticin 1. Exclusively coupled to the G(q) subclass of heteromeric G proteins. Activation leads to mobilization of calcium, stimulation of phosphoinositide turnover and activation of p44/p42 mitogen-activated protein kinase. May play a role during early pregnancy ().
Research Articles on PROKR1
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Product Notes
The Mouse PROKR1 prokr1 (Catalog #AAA9717259) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717259 ELISA Kit recognizes Mouse PROKR1. It is sometimes possible for the material contained within the vial of "Prokineticin Receptor 1 (PROKR1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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