Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Glycogen phosphorylase, muscle form (PYGM) Standard Curve.)
Mouse Glycogen Phosphorylase, Muscle Form (PYGM) ELISA Kit | PYGM elisa kit
Mouse Glycogen Phosphorylase, Muscle Form (PYGM) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Glycogen phosphorylase, muscle form (PYGM) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Glycogen phosphorylase, muscle form (PYGM) Standard Curve.)
Background: Mutations in the muscle isoform of glycogen phosphorylase (PYGM) are associated with McArdle disease (glycogen storage disease type V). More than 65 mutations in the PYGM gene that lead to McArdle disease have been identified to date.Gautron et al. (1987) isolated muscle phosphorylase cDNA clones from a human cDNA library. Northern blot experiments revealed 1 specific mRNA of 3.4 kb found uniquely in tissues expressing muscle phosphorylase. The muscle glycogen phosphorylase protein comprises 842 amino acids (Kubisch et al., 1998).Burke et al. (1987) determined the intron/exon structure of the PYGM gene. Kubisch et al. (1998) provided a revised genomic structure for the PYGM gene, which contains 20 exons.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a glycolysis enzyme found in muscle. Highly similar enzymes encoded by different genes are found in liver and brain. The encoded protein is involved in regulating the breakdown of glycogen to glucose-1-phosphate, which is necessary for ATP generation. [provided by RefSeq, Dec 2015]
Uniprot Description
PYGM: an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Dimers associate into a tetramer to form the enzymatically active phosphorylase A. Phosphorylation of Ser-14 converts phosphorylase B (unphosphorylated) to phosphorylase A.
Protein type: Phosphorylase; Transferase; Endoplasmic reticulum; Carbohydrate Metabolism - starch and sucrose; EC 2.4.1.1
Cellular Component: sarcoplasmic reticulum; cytoplasm; Z disc
Molecular Function: transferase activity; transferase activity, transferring glycosyl groups; glycogen phosphorylase activity; phosphorylase activity; nucleotide binding; drug binding; carbohydrate binding; catalytic activity; pyridoxal phosphate binding; AMP binding
Biological Process: cellular calcium ion homeostasis; glycogen metabolic process; response to organic substance; response to cAMP; glycogen catabolic process; metabolic process; carbohydrate metabolic process; response to hypoxia
Research Articles on PYGM
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Product Notes
The Mouse PYGM pygm (Catalog #AAA9717198) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717198 ELISA Kit recognizes Mouse PYGM. It is sometimes possible for the material contained within the vial of "Glycogen Phosphorylase, Muscle Form (PYGM), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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