Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Glycogen phosphorylase (GP) Standard Curve.)
Rat Glycogen Phosphorylase (GP) ELISA Kit | GP elisa kit
Rat Glycogen Phosphorylase (GP) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Glycogen phosphorylase (GP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Glycogen phosphorylase (GP) Standard Curve.)
Background: Glycogen phosphorylase is one of the phosphorylase enzymes (EC 2.4.1.1). Glycogen phosphorylase catalyzes the rate-limiting step in glycogenolysis in animals by releasing glucose-1-phosphate from the terminal alpha-1,4-glycosidic bond. Glycogen phosphorylase is also studied as a model protein regulated by both reversible phosphorylation and allosteric effects
NCBI and Uniprot Product Information
NCBI Description
catalyzes the rate-limiting step of glycogen catabolism [RGD, Feb 2006]
Uniprot Description
PYGL: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGL are the cause of glycogen storage disease type 6 (GSD6). A metabolic disorder characterized by mild to moderate hypoglycemia, mild ketosis, growth retardation, and prominent hepatomegaly. Heart and skeletal muscle are not affected. Belongs to the glycogen phosphorylase family.
Protein type: Carbohydrate Metabolism - starch and sucrose; EC 2.4.1.1; Transferase
Chromosomal Location of Human Ortholog: 6q24
Cellular Component: cytoplasm
Molecular Function: AMP binding; ATP binding; bile acid binding; carbohydrate binding; drug binding; glycogen phosphorylase activity; protein homodimerization activity; purine binding; pyridoxal phosphate binding; vitamin binding
Biological Process: 5-phosphoribose 1-diphosphate biosynthetic process; glucose homeostasis; glycogen catabolic process; glycogen metabolic process
Research Articles on GP
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Product Notes
The Rat GP pygl (Catalog #AAA9712003) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712003 ELISA Kit recognizes Rat GP. It is sometimes possible for the material contained within the vial of "Glycogen Phosphorylase (GP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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