Rabbit anti-Human FUT6 Polyclonal Antibody | anti-FUT6 antibody

FUT6, NT (FUT6, FCT3A, Alpha-(1,3)-fucosyltransferase, Fucosyltransferase 6, Fucosyltransferase VI, Galactoside 3-L-fucosyltransferase) (APC)

Cat. Num. AAA6300412

• Gene Names: FUT6; FT1A; FCT3A; Fuc-TVI; FucT-VI
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FUT6; Polyclonal Antibody; NT (FUT6; FCT3A; Alpha-(1; 3)-fucosyltransferase; Fucosyltransferase 6; Fucosyltransferase VI; Galactoside 3-L-fucosyltransferase) (APC); Galactoside 3-L-fucosyltransferase; anti-FUT6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 359

• Applicable Applications for anti-FUT6 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: FUT6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-49 amino acids from the N-terminal region of human FUT6.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(FUT6 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FUT6 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(FUT6 Antibody (N-term) western blot analysis in ZR-75-1,NCI-H292,293 cell line lysates (35ug/lane).This demonstrates the FUT6 antibody detected the FUT6 protein (arrow).)

Related Product Information for anti-FUT6 antibody

The protein encoded by this gene is a Golgi stack membrane protein that is involved in the creation of sialyl-Lewis X, an E-selectin ligand. Mutations in this gene are a cause of fucosyltransferase-6 deficiency. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-FUT6 antibody

Antibodies; Enzymes; Glycosyltransferase

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Stern, H.M., et al. Clin. Cancer Res. 16(5):1587-1596(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Norden, R., et al. Glycobiology 19(7):776-788(2009). Higai, K., et al. Glycoconj. J. 25(3):225-235(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 103472033
• NCBI GeneID: 2528
• NCBI Accession #: NP_001035791.1
• NCBI GenBank Nucleotide #: NP_001035791.1
• UniProt Accession #: P51993
• NCBI Official Full Name: alpha-(1,3)-fucosyltransferase 6
• NCBI Official Synonym Full Names: fucosyltransferase 6
• NCBI Official Symbol: FUT6
• NCBI Official Synonym Symbols: FT1A; FCT3A; Fuc-TVI; FucT-VI
• NCBI Protein Information: alpha-(1,3)-fucosyltransferase 6
• UniProt Protein Name: Alpha-(1,3)-fucosyltransferase 6
• Protein Family: Fucosyltransferase
• UniProt Gene Name: FUT6
• UniProt Synonym Gene Names: FCT3A; Fuc-TVI; FucT-VI
• UniProt Entry Name: FUT6_HUMAN

NCBI Description

The protein encoded by this gene is a Golgi stack membrane protein that is involved in the creation of sialyl-Lewis X, an E-selectin ligand. Mutations in this gene are a cause of fucosyltransferase-6 deficiency. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FUT6: Enzyme involved in the biosynthesis of the E-Selectin ligand, sialyl-Lewis X. Catalyzes the transfer of fucose from GDP- beta-fucose to alpha-2,3 sialylated substrates. Belongs to the glycosyltransferase 10 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.4.1.65; Membrane protein, integral; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: Golgi apparatus; integral to membrane

Molecular Function: 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase activity; alpha(1,3)-fucosyltransferase activity; fucosyltransferase activity

Biological Process: protein amino acid glycosylation; L-fucose catabolic process

Disease: Fucosyltransferase 6 Deficiency