Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Sodium/glucose cotransporter 1 (SLC5A1) Standard Curve.)
Mouse Sodium/Glucose Cotransporter 1 (SLC5A1) ELISA Kit | SLC5A1 elisa kit
Mouse Sodium/Glucose Cotransporter 1 (SLC5A1) ELISA Kit
Gene Names
Slc5a1; Sglt1
Reactivity
Mouse
Synonyms
Sodium/Glucose Cotransporter 1 (SLC5A1); Mouse Sodium/Glucose Cotransporter 1 (SLC5A1) ELISA Kit; SLC5A1; RP1-127L4.1; D22S675; NAGT; SGLT1; Human Na+/glucose cotransporter 1 mRNA; complete cds; sodium/glucose cotransporter 1; solute carrier family 5 (sodium/glucose transporter); member 1; solute carrier f; SLC5A1 elisa kit
Reactivity
Mouse
Specificity
Mouse Sodium/glucose cotransporter 1 (SLC5A1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SLC5A1. No significant cross-reactivity or interference between Mouse SLC5A1 and analogues was observed.
Sequence Length
665
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Sodium/glucose cotransporter 1 (SLC5A1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Sodium/glucose cotransporter 1 (SLC5A1) Standard Curve.)
Related Product Information for SLC5A1 elisa kit
Description: This Mouse Sodium/glucose cotransporter 1 (SLC5A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC5A1 in samples. An antibody specific for SLC5A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC5A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC5A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC5A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Glucose transporters are integral membrane proteins that mediate the transport of glucose and structurally-related substances across cellular membranes. Two families of glucose transporter have been identified: the facilitated diffusion glucose transporter family (GLUT family), also known as 'uniporters,' and the sodium-dependent glucose transporter family (SGLT family), also known as 'cotransporters' or 'symporters'. The SLC5A1 gene encodes a protein that is involved in the active transport of glucose and galactose into eukaryotic and some prokaryotic cells.The SLC5A1 protein contains a core of 13 transmembrane domains, which it shares with other members of the gene family, and it contains an additional transmembrane appended to the C termin.
Background: Glucose transporters are integral membrane proteins that mediate the transport of glucose and structurally-related substances across cellular membranes. Two families of glucose transporter have been identified: the facilitated diffusion glucose transporter family (GLUT family), also known as 'uniporters,' and the sodium-dependent glucose transporter family (SGLT family), also known as 'cotransporters' or 'symporters'. The SLC5A1 gene encodes a protein that is involved in the active transport of glucose and galactose into eukaryotic and some prokaryotic cells.The SLC5A1 protein contains a core of 13 transmembrane domains, which it shares with other members of the gene family, and it contains an additional transmembrane appended to the C termin.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
sodium/glucose cotransporter 1
NCBI Official Synonym Full Names
solute carrier family 5 (sodium/glucose cotransporter), member 1
NCBI Official Symbol
Slc5a1
NCBI Official Synonym Symbols
Sglt1
NCBI Protein Information
sodium/glucose cotransporter 1
UniProt Protein Name
Sodium/glucose cotransporter 1
Protein Family
UniProt Gene Name
Slc5a1
UniProt Synonym Gene Names
Na(+)/glucose cotransporter 1
Uniprot Description
Actively transports glucose into cells by Na+ cotransport with a Na+ to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na+/glucose cotransporter arranged in series along kidney proximal tubules.
Research Articles on SLC5A1
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Product Notes
The Mouse SLC5A1 slc5a1 (Catalog #AAA9717030) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717030 ELISA Kit recognizes Mouse SLC5A1. It is sometimes possible for the material contained within the vial of "Sodium/Glucose Cotransporter 1 (SLC5A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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