Mouse Sodium/Glucose Cotransporter 1 (SGLT1) ELISA Kit | SGLT1 elisa kit

Mouse Sodium/Glucose Cotransporter 1 (SGLT1) ELISA Kit

Cat. Num. AAA2024028

• Gene Names: Slc5a1; Sglt1
• Reactivity: Mouse
• Synonyms: Sodium/Glucose Cotransporter 1 (SGLT1); Mouse Sodium/Glucose Cotransporter 1 (SGLT1) ELISA Kit; SLC5A1; NAGT; Solute Carrier Family 5 Member 1; High affinity sodium-glucose cotransporter; Na(+)/glucose cotransporter 1; SGLT1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sodium/Glucose Cotransporter 1 (SGLT1).; No significant cross-reactivity or interference between Sodium/Glucose Cotransporter 1 (SGLT1) and analogues was observed.
• Sequence Length: 665

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sodium/Glucose Cotransporter 1 (SGLT1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sodium/Glucose Cotransporter 1 (SGLT1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SGLT1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sodium/Glucose Cotransporter 1 (SGLT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sodium/Glucose Cotransporter 1 (SGLT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sodium/Glucose Cotransporter 1 (SGLT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sodium/Glucose Cotransporter 1 (SGLT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SGLT1 elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 261824023
• NCBI GeneID: 20537
• NCBI Accession #: NP_062784.3
• NCBI GenBank Nucleotide #: NM_019810.4
• UniProt Accession #: Q8C3K6
• Molecular Weight: 73,449 Da
• NCBI Official Full Name: sodium/glucose cotransporter 1
• NCBI Official Synonym Full Names: solute carrier family 5 (sodium/glucose cotransporter), member 1
• NCBI Official Symbol: Slc5a1
• NCBI Official Synonym Symbols: Sglt1
• NCBI Protein Information: sodium/glucose cotransporter 1
• UniProt Protein Name: Sodium/glucose cotransporter 1
• UniProt Gene Name: Slc5a1
• UniProt Synonym Gene Names: Na(+)/glucose cotransporter 1
• UniProt Entry Name: SC5A1_MOUSE

Uniprot Description

SLC5A1: Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. Expressed mainly in intestine and kidney. Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

Protein type: Membrane protein, integral; Transporter, SLC family; Transporter; Membrane protein, multi-pass

Cellular Component: membrane; brush border membrane; apical plasma membrane; integral to membrane; intercellular junction; brush border

Molecular Function: glucose:sodium symporter activity; transporter activity; symporter activity; glucose transmembrane transporter activity

Biological Process: transport; sodium ion transport; carbohydrate transport; ion transport; glucose transport; transmembrane transport; metanephros development; intestinal absorption