Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Spectrin alpha chain, brain (SPTAN1) Standard Curve.)
Mouse Spectrin alpha Chain, Brain (SPTAN1) ELISA Kit | SPTAN1 elisa kit
Mouse Spectrin alpha Chain, Brain (SPTAN1) ELISA Kit
Gene Names
Sptan1; Spna2; Spna-2; 2610027H02Rik
Reactivity
Mouse
Synonyms
Spectrin alpha Chain; Brain (SPTAN1); Mouse Spectrin alpha Chain; Brain (SPTAN1) ELISA Kit; SPTAN1; (ALPHA)II-SPECTRIN; FLJ44613; NEAS; non-erythrocytic spectrin alpha; SPTAN1 elisa kit
Reactivity
Mouse
Specificity
Mouse Spectrin alpha chain, brain (SPTAN1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPTAN1. No significant cross-reactivity or interference between Mouse SPTAN1 and analogues was observed.
Sequence Length
2472
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Spectrin alpha chain, brain (SPTAN1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Spectrin alpha chain, brain (SPTAN1) Standard Curve.)
Related Product Information for SPTAN1 elisa kit
Description: This Mouse Spectrin alpha chain, brain (SPTAN1) ELISA Kit employs a two-site sandwich ELISA to quantitate SPTAN1 in samples. An antibody specific for SPTAN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPTAN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPTAN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPTAN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Alpha-Fodrin is an intracellular, actin-binding, organ-specific protein of the cytoskeleton. The network of actin and fodrin situated below the plasma membrane of secretorial cells, is important for the alignment of secretorial vesicles to the plasma membrane during secretorial processes. During apoptosis the alpha-fodrin dimer is cleaved into a 120 kDa breakdown product, which is found abundantly in the salivary gland. This proteolysis of fodrin may be a consequence of protease activation during apoptosis. The cleavage product of 120 kDa alpha-fodrin was found to be an important autoantigen in the pathogenesis of organ-specific autoimmune esponse. Clinical studies have shown, that in patients with Sjögren Syndrome alpha-fodrin is involved in the stimulation of peripheral blood T-cells.
Background: Alpha-Fodrin is an intracellular, actin-binding, organ-specific protein of the cytoskeleton. The network of actin and fodrin situated below the plasma membrane of secretorial cells, is important for the alignment of secretorial vesicles to the plasma membrane during secretorial processes. During apoptosis the alpha-fodrin dimer is cleaved into a 120 kDa breakdown product, which is found abundantly in the salivary gland. This proteolysis of fodrin may be a consequence of protease activation during apoptosis. The cleavage product of 120 kDa alpha-fodrin was found to be an important autoantigen in the pathogenesis of organ-specific autoimmune esponse. Clinical studies have shown, that in patients with Sjögren Syndrome alpha-fodrin is involved in the stimulation of peripheral blood T-cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Spectrin alpha chain, non-erythrocytic 1
NCBI Official Synonym Full Names
spectrin alpha, non-erythrocytic 1
NCBI Official Symbol
Sptan1
NCBI Official Synonym Symbols
Spna2; Spna-2; 2610027H02Rik
NCBI Protein Information
spectrin alpha chain, non-erythrocytic 1
UniProt Protein Name
Spectrin alpha chain, non-erythrocytic 1
Protein Family
UniProt Gene Name
Sptan1
UniProt Synonym Gene Names
Spna2; Spta2
Uniprot Description
Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Research Articles on SPTAN1
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Product Notes
The Mouse SPTAN1 sptan1 (Catalog #AAA9716858) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716858 ELISA Kit recognizes Mouse SPTAN1. It is sometimes possible for the material contained within the vial of "Spectrin alpha Chain, Brain (SPTAN1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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