Rabbit SPTAN1 Polyclonal Antibody | anti-SPTAN1 antibody

SPTAN1 Polyclonal Antibody

Cat. Num. AAA3007588

• Gene Names: SPTAN1; NEAS; EIEE5; SPTA2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SPTAN1; Polyclonal Antibody; SPTAN1 Polyclonal Antibody; Spectrin alpha chain; non-erythrocytic 1; Alpha-II spectrin; Fodrin alpha chain; Spectrin; non-erythroid alpha subunit; NEAS; SPTA2; anti-SPTAN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SPTAN1 polyclonal antibody detects endogenous levels of SPTAN1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 2477

• Applicable Applications for anti-SPTAN1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding Human SPTAN1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SPTAN1 polyclonal antibody at 1:500 dilutionLane1:L02 whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug))

Related Product Information for anti-SPTAN1 antibody

Fodrin (also named nonerythroid spectrin) is a universally expressed membrane-associated cytoskeletal protein consisting of alpha- and beta-subunits. This protein is important for maintaining normal membrane structure and supporting cell surface protein function. Alpha-fodrin is one of the primary targets cleaved by caspases during apoptosis. The full length 240 kDa protein can be cleaved at several sites within its sequence by activated caspases to yield amino-terminal 150 kDa, carboxy-terminal 120 kDa and 35 kDa major products. Cleavage of alpha-fodrin leads to membrane malfunction and cell shrinkage.

NCBI and Uniprot Product Information

• NCBI GI #: 194595509
• NCBI GeneID: 6709
• NCBI Accession #: NP_001123910.1
• NCBI GenBank Nucleotide #: NP_001123910.1
• UniProt Accession #: Q13813
• Molecular Weight: ~ 150kDa
• NCBI Official Full Name: spectrin alpha chain, non-erythrocytic 1 isoform 1
• NCBI Official Synonym Full Names: spectrin alpha, non-erythrocytic 1
• NCBI Official Symbol: SPTAN1
• NCBI Official Synonym Symbols: NEAS; EIEE5; SPTA2
• NCBI Protein Information: spectrin alpha chain, non-erythrocytic 1
• UniProt Protein Name: Spectrin alpha chain, non-erythrocytic 1
• Protein Family: Spectrin
• UniProt Gene Name: SPTAN1
• UniProt Synonym Gene Names: NEAS; SPTA2
• UniProt Entry Name: SPTN1_HUMAN

NCBI Description

Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

Uniprot Description

SPTAN1: a cytoskeletal protein which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. Capable to forming dimers which can further associate to tetramers.

Protein type: Adaptor/scaffold; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: spectrin; microtubule cytoskeleton; membrane; intracellular membrane-bound organelle; fascia adherens; cytosol; Z disc; vesicle; lateral plasma membrane

Molecular Function: calmodulin binding; protein binding; spectrin binding; structural constituent of cytoskeleton; protein heterodimerization activity; calcium ion binding; actin binding

Biological Process: axon guidance; apoptosis; actin filament capping; cell structure disassembly during apoptosis

Disease: Epileptic Encephalopathy, Early Infantile, 5