Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tumor necrosis factor ligand superfamily member 14 (TNFSF14) Standard Curve.)
Mouse Tumor Necrosis Factor Ligand Superfamily Member 14 (TNFSF14) ELISA Kit | TNFSF14 elisa kit
Mouse Tumor Necrosis Factor Ligand Superfamily Member 14 (TNFSF14) ELISA Kit
Gene Names
Tnfsf14; LTg; HVEML; LIGHT; Ly113; HVEM-L; Tnlg1d
Reactivity
Mouse
Synonyms
Tumor Necrosis Factor Ligand Superfamily Member 14 (TNFSF14); Mouse Tumor Necrosis Factor Ligand Superfamily Member 14 (TNFSF14) ELISA Kit; TNFSF14; CD258; HVEML; LIGHT; LTg; TR2; delta transmembrane LIGHT; herpesvirus entry mediator A; ligand for herpesvirus entry mediator; tumor necrosis factor ligand superfamily; member 14; tumor necrosis factor; TNFSF14 elisa kit
Reactivity
Mouse
Specificity
Mouse Tumor necrosis factor ligand superfamily member 14 (TNFSF14) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNFSF14. No significant cross-reactivity or interference between Mouse TNFSF14 and analogues was observed.
Sequence Length
239
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tumor necrosis factor ligand superfamily member 14 (TNFSF14) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tumor necrosis factor ligand superfamily member 14 (TNFSF14) Standard Curve.)
Related Product Information for TNFSF14 elisa kit
Description: This Mouse Tumor necrosis factor ligand superfamily member 14 (TNFSF14) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF14 in samples. An antibody specific for TNFSF14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFSF14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Tumor necrosis factor ligand superfamily member 14 is a protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.
Background: Tumor necrosis factor ligand superfamily member 14 is a protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 14
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 14
NCBI Official Symbol
Tnfsf14
NCBI Official Synonym Symbols
LTg; HVEML; LIGHT; Ly113; HVEM-L; Tnlg1d
NCBI Protein Information
tumor necrosis factor ligand superfamily member 14
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 14
Protein Family
UniProt Gene Name
Tnfsf14
UniProt Synonym Gene Names
Light
UniProt Entry Name
TNF14_MOUSE
Uniprot Description
TNFSF14: Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytokine; Inhibitor; Membrane protein, integral
Cellular Component: extracellular space; membrane; integral to membrane; extracellular region; plasma membrane
Molecular Function: caspase inhibitor activity; cytokine activity; tumor necrosis factor receptor binding; receptor binding
Biological Process: release of cytoplasmic sequestered NF-kappaB; T cell costimulation; immune response; negative regulation of caspase activity; positive regulation of myoblast differentiation
Research Articles on TNFSF14
Similar Products
Product Notes
The Mouse TNFSF14 tnfsf14 (Catalog #AAA9716725) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716725 ELISA Kit recognizes Mouse TNFSF14. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Ligand Superfamily Member 14 (TNFSF14), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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