Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Enkephalin (ENK) Standard Curve.)
Human Enkephalin (ENK) ELISA Kit | ENK elisa kit
Human Enkephalin (ENK) ELISA Kit
Gene Names
Penk; Enk; Penk1; Penk2; Penk-rs
Reactivity
Human
Synonyms
Enkephalin (ENK); Human Enkephalin (ENK) ELISA Kit; ENK elisa kit
Reactivity
Human
Specificity
Human Enkephalin (ENK) ELISA Kit has high sensitivity and excellent specificity for detection of Human ENK. No significant cross-reactivity or interference between Human ENK and analogues was observed.
Sequence Length
269
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Enkephalin (ENK) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Enkephalin (ENK) Standard Curve.)
Related Product Information for ENK elisa kit
Description: This Human Enkephalin (ENK) ELISA Kit employs a two-site sandwich ELISA to quantitate ENK in samples. An antibody specific for ENK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ENK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ENK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ENK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: An enkephalin is a pentapeptide ending with either leucine ("leu") or methionine ("met"). Both are products of the proenkephalin gene. Enkephalins play many roles in regulating pain. Met-enkephalin is an endogenous opioid peptide neurotransmitter found naturally in many part of the animal and human's body. One of the primary locations is in segment II of the dorsal horn of the spinal cord. Also present in the CNS (central nervous system), it is one of the two forms of enkephalin; the other is leu-enkephalin. The tyrosine residue at position 1 is thought to be analogous to the 3-hydroxyl group on morphine. Leu-enkephalin is an endogenous opioid peptide neurotransmitter found naturally in the brains of many animals, including humans. It is one of the two forms of enkephalin; the other is met-enkephalin. The tyrosine residue at position 1 is thought to be analogous to the 3-hydroxyl group on morphine.
Background: An enkephalin is a pentapeptide ending with either leucine ("leu") or methionine ("met"). Both are products of the proenkephalin gene. Enkephalins play many roles in regulating pain. Met-enkephalin is an endogenous opioid peptide neurotransmitter found naturally in many part of the animal and human's body. One of the primary locations is in segment II of the dorsal horn of the spinal cord. Also present in the CNS (central nervous system), it is one of the two forms of enkephalin; the other is leu-enkephalin. The tyrosine residue at position 1 is thought to be analogous to the 3-hydroxyl group on morphine. Leu-enkephalin is an endogenous opioid peptide neurotransmitter found naturally in the brains of many animals, including humans. It is one of the two forms of enkephalin; the other is met-enkephalin. The tyrosine residue at position 1 is thought to be analogous to the 3-hydroxyl group on morphine.
NCBI and Uniprot Product Information
NCBI Description
neuropeptide hormone; plays a role in the responses to stress and pain perception [RGD, Feb 2006]
Research Articles on ENK
Similar Products
Product Notes
The Human ENK (Catalog #AAA9716295) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716295 ELISA Kit recognizes Human ENK. It is sometimes possible for the material contained within the vial of "Enkephalin (ENK), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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