Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Soluble cluster of differentiation 14 (sCD14) Standard Curve.)
Human Soluble Cluster Of Differentiation 14 (sCD14) ELISA Kit | sCD14 elisa kit
Human Soluble Cluster Of Differentiation 14 (sCD14) ELISA Kit
Reactivity
Human
Synonyms
Soluble Cluster Of Differentiation 14 (sCD14); Human Soluble Cluster Of Differentiation 14 (sCD14) ELISA Kit; CD14; CD14 antigen; monocyte differentiation antigen CD14; sCD14 elisa kit
Reactivity
Human
Specificity
Human Soluble cluster of differentiation 14 (sCD14) ELISA Kit has high sensitivity and excellent specificity for detection of Human CD14. No significant cross-reactivity or interference between Human CD14 and analogues was observed.
Sequence Length
375
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Soluble cluster of differentiation 14 (sCD14) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Soluble cluster of differentiation 14 (sCD14) Standard Curve.)
Related Product Information for sCD14 elisa kit
Description: This Human Soluble cluster of differentiation 14 (sCD14) ELISA Kit employs a two-site sandwich ELISA to quantitate CD14 in samples. An antibody specific for CD14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: CD14 exists in two forms. It is either anchored into the membrane by a glycosylphosphatidylinositol tail (mCD14) or it appears in a soluble form (sCD14). Soluble CD14 appears either after shedding of mCD14 (48 KDa) or is directly secreted from intracellular vesicles (56 KDa).CD14 takes its name from its inclusion in the cluster of differentiation group of cell surface marker proteins. CD14 was the first described pattern recognition receptor.CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can only bind LPS in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand CD14 also recognizes other pathogen associated molecular patterns.
Background: CD14 exists in two forms. It is either anchored into the membrane by a glycosylphosphatidylinositol tail (mCD14) or it appears in a soluble form (sCD14). Soluble CD14 appears either after shedding of mCD14 (48 KDa) or is directly secreted from intracellular vesicles (56 KDa).CD14 takes its name from its inclusion in the cluster of differentiation group of cell surface marker proteins. CD14 was the first described pattern recognition receptor.CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can only bind LPS in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand CD14 also recognizes other pathogen associated molecular patterns.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
monocyte differentiation antigen CD14
NCBI Official Synonym Full Names
CD14 molecule
NCBI Official Symbol
CD14
NCBI Protein Information
monocyte differentiation antigen CD14
UniProt Protein Name
Monocyte differentiation antigen CD14
UniProt Gene Name
CD14
UniProt Entry Name
CD14_HUMAN
NCBI Description
The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]
Research Articles on sCD14
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Product Notes
The Human sCD14 cd14 (Catalog #AAA9715947) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715947 ELISA Kit recognizes Human sCD14. It is sometimes possible for the material contained within the vial of "Soluble Cluster Of Differentiation 14 (sCD14), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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