Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Krueppel-like factor 2 (KLF2) Standard Curve.)
Human Krueppel-Like Factor 2 (KLF2) ELISA Kit | KLF2 elisa kit
Human Krueppel-Like Factor 2 (KLF2) ELISA Kit
Gene Names
KLF2; LKLF
Reactivity
Human
Synonyms
Krueppel-Like Factor 2 (KLF2); Human Krueppel-Like Factor 2 (KLF2) ELISA Kit; KLF2; LKLF; Kruppel-like factor; Kruppel-like factor LKLF; lung Kruppel-like zinc finger transcription factor; KLF2 elisa kit
Reactivity
Human
Specificity
Human Krueppel-like factor 2 (KLF2) ELISA Kit has high sensitivity and excellent specificity for detection of Human KLF2. No significant cross-reactivity or interference between Human KLF2 and analogues was observed.
Sequence Length
355
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Krueppel-like factor 2 (KLF2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Krueppel-like factor 2 (KLF2) Standard Curve.)
Related Product Information for KLF2 elisa kit
Description: This Human Krueppel-like factor 2 (KLF2) ELISA Kit employs a two-site sandwich ELISA to quantitate KLF2 in samples. An antibody specific for KLF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Mature single-positive (CD4+ or CD8+) T lymphocytes enter a 'resting' state in which they are proliferatively quiescent and relatively resistant to apoptosis. Anderson et al. (1995) identified and characterized the gene for a novel zinc finger transcription factor that they symbolized LKLF for 'lung Kruppel-like factor.' Using 5-prime and 3-prime RACE on a melted GC-rich DNA fragment similar to mouse Lklf, Kozyrev et al. (1999) isolated a cDNA encoding KLF2, which they termed LKLF. Sequence analysis predicted that the 355-amino acid LKLF protein, which is 87% identical to the mouse protein, has N-terminal proline-rich repeats, a putative activation domain, a potential nuclear localization signal, and a C-terminal zinc finger domain.
Background: Mature single-positive (CD4+ or CD8+) T lymphocytes enter a 'resting' state in which they are proliferatively quiescent and relatively resistant to apoptosis. Anderson et al. (1995) identified and characterized the gene for a novel zinc finger transcription factor that they symbolized LKLF for 'lung Kruppel-like factor.' Using 5-prime and 3-prime RACE on a melted GC-rich DNA fragment similar to mouse Lklf, Kozyrev et al. (1999) isolated a cDNA encoding KLF2, which they termed LKLF. Sequence analysis predicted that the 355-amino acid LKLF protein, which is 87% identical to the mouse protein, has N-terminal proline-rich repeats, a putative activation domain, a potential nuclear localization signal, and a C-terminal zinc finger domain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
Krueppel-like factor 2
NCBI Official Synonym Full Names
Kruppel like factor 2
NCBI Official Symbol
KLF2
NCBI Official Synonym Symbols
LKLF
NCBI Protein Information
Krueppel-like factor 2
UniProt Protein Name
Krueppel-like factor 2
Protein Family
UniProt Gene Name
KLF2
UniProt Synonym Gene Names
LKLF
NCBI Description
This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is expressed early in mammalian development and is found in many different cell types. The protein acts to bind the CACCC box found in the promoter of target genes to activate their transcription. It plays a role in many processes during development and disease including adipogenesis, embryonic erythropoiesis, epithelial integrity, inflammation and t-cell viability. [provided by RefSeq, Mar 2017]
Uniprot Description
Transcription factor that binds to the CACCC box in the promoter of target genes such as HBB/beta globin or NOV and activates their transcription.
Research Articles on KLF2
Similar Products
Product Notes
The Human KLF2 klf2 (Catalog #AAA9715903) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715903 ELISA Kit recognizes Human KLF2. It is sometimes possible for the material contained within the vial of "Krueppel-Like Factor 2 (KLF2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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