Mouse Krueppel-Like Factor 1 (KLF1) ELISA Kit | KLF1 elisa kit

Mouse Krueppel-Like Factor 1 (KLF1) ELISA Kit

Cat. Num. AAA9717816

• Reactivity: Mouse
• Synonyms: Krueppel-Like Factor 1 (KLF1); Mouse Krueppel-Like Factor 1 (KLF1) ELISA Kit; KLF1; EKLF; INLU; erythroid Kruppel-like factor; erythroid-specific transcription factor EKLF; monoclonal antibody A3D8; KLF1 elisa kit

• Reactivity: Mouse
• Specificity: Mouse Krueppel-like factor 1 (KLF1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse KLF1. No significant cross-reactivity or interference between Mouse KLF1 and analogues was observed.
• Sequence Length: 358

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Krueppel-like factor 1 (KLF1) Standard Curve.)

Related Product Information for KLF1 elisa kit

Description: This Mouse Krueppel-like factor 1 (KLF1) ELISA Kit employs a two-site sandwich ELISA to quantitate KLF1 in samples. An antibody specific for KLF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Erythroid Krüppel-like Factor (EKLF) is a transcription factor that is necessary for the proper maturation of erythroid (red blood) cells. The molecule has two domains; the transactivation domain and the chromatin-remodeling domain. The carboxyl (C) terminal is composed of three C2H2 zinc fingers that binds to DNA, and the amino (N) terminus is proline rich and acidic. The gene for EKLF is on the human chromosome 19 and on mouse chromosome 8. EKLF deficient (knockout) mouse embryos exhibit a lethal anemic phenotype, fail to promote the transcription of adult beta globin, and die by embryonic day 14. On the other hand, over-expression of EKLF results in a reduction of the number of circulating platelets and hastens the onset of beta globin gene.

NCBI and Uniprot Product Information

• NCBI GI #: 2507549
• UniProt Accession #: P46099
• NCBI Official Full Name: Krueppel-like factor 1
• UniProt Protein Name: Krueppel-like factor 1
• Protein Family: Krueppel-like factor
• UniProt Gene Name: Klf1
• UniProt Synonym Gene Names: Elkf; EKLF

Uniprot Description

Transcription regulator of erythrocyte development. Binds to the CACCC box in the beta-globin gene promoter and activates transcription. Probably serves as a general switch factor for erythroid development. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.