Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human MAD2L1-binding protein (MAD2L1BP) Standard Curve.)
Human MAD2L1-Binding Protein (MAD2L1BP) ELISA Kit | MAD2L1BP elisa kit
Human MAD2L1-Binding Protein (MAD2L1BP) ELISA Kit
Gene Names
MAD2L1BP; CMT2
Reactivity
Human
Synonyms
MAD2L1-Binding Protein (MAD2L1BP); Human MAD2L1-Binding Protein (MAD2L1BP) ELISA Kit; MAD2L1BP; CMT2; KIAA0110; MGC11282; RP1-261G23.6; caught by MAD2 protein; MAD2L1BP elisa kit
Reactivity
Human
Specificity
Human MAD2L1-binding protein (MAD2L1BP) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAD2L1BP. No significant cross-reactivity or interference between Human MAD2L1BP and analogues was observed.
Sequence Length
306
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human MAD2L1-binding protein (MAD2L1BP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human MAD2L1-binding protein (MAD2L1BP) Standard Curve.)
Related Product Information for MAD2L1BP elisa kit
Description: This Human MAD2L1-binding protein (MAD2L1BP) ELISA Kit employs a two-site sandwich ELISA to quantitate MAD2L1BP in samples. An antibody specific for MAD2L1BP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAD2L1BP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAD2L1BP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAD2L1BP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MAD2L1BPwas identified as a binding protein of the MAD2 mitotic arrest deficient-like 1 (MAD2/MAD2L1). This protein may interact with the spindle checkpoint and coordinate cell cycle events in late mitosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. Upon the completion of spindle attachment, formation of the CMT2-MAD2 complex coincides with dissociation of the p55CDC-MAD2 complex. Overexpression of CMT2 in cells arrested by the spindle checkpoint causes premature destruction of securin and allows exit from mitosis without chromosome segregation. Depletion of CMT2 induces cell death following a transient delay in the onset of anaphase. These results indicate that CMT3 interacts with the spindle checkpoint and coordinates cell cycle events in late mitosis.
Background: MAD2L1BPwas identified as a binding protein of the MAD2 mitotic arrest deficient-like 1 (MAD2/MAD2L1). This protein may interact with the spindle checkpoint and coordinate cell cycle events in late mitosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. Upon the completion of spindle attachment, formation of the CMT2-MAD2 complex coincides with dissociation of the p55CDC-MAD2 complex. Overexpression of CMT2 in cells arrested by the spindle checkpoint causes premature destruction of securin and allows exit from mitosis without chromosome segregation. Depletion of CMT2 induces cell death following a transient delay in the onset of anaphase. These results indicate that CMT3 interacts with the spindle checkpoint and coordinates cell cycle events in late mitosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
MAD2L1-binding protein isoform 1
NCBI Official Synonym Full Names
MAD2L1 binding protein
NCBI Official Symbol
MAD2L1BP
NCBI Official Synonym Symbols
CMT2
NCBI Protein Information
MAD2L1-binding protein
UniProt Protein Name
MAD2L1-binding protein
Protein Family
UniProt Gene Name
MAD2L1BP
UniProt Synonym Gene Names
CMT2; KIAA0110
UniProt Entry Name
MD2BP_HUMAN
NCBI Description
The protein encoded by this gene was identified as a binding protein of the MAD2 mitotic arrest deficient-like 1 (MAD2/MAD2L1). MAD2 is a key component of the spindle checkpoint that delays the onset of anaphase until all the kinetochores are attached to the spindle. This protein may interact with the spindle checkpoint and coordinate cell cycle events in late mitosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Uniprot Description
MAD2L1BP: May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. Belongs to the MAD2L1BP family.
Protein type: Cell cycle regulation
Chromosomal Location of Human Ortholog: 6p21.1
Cellular Component: intracellular membrane-bound organelle; cytoplasm; nucleolus; spindle; nucleus
Molecular Function: protein binding
Biological Process: mitotic cell cycle checkpoint; regulation of exit from mitosis
Research Articles on MAD2L1BP
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Product Notes
The Human MAD2L1BP mad2l1bp (Catalog #AAA9715422) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715422 ELISA Kit recognizes Human MAD2L1BP. It is sometimes possible for the material contained within the vial of "MAD2L1-Binding Protein (MAD2L1BP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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