Rat IGFBP-4 ELISA Kit | IGFBP-4 elisa kit

Rat IGFBP-4 (Insulin-Like Growth Factor Binding Protein 4) ELISA Kit

Cat. Num. AAA2502844

• Gene Names: IGFBP4; BP-4; IBP4; IGFBP-4; HT29-IGFBP
• Reactivity: Rat
• Synonyms: IGFBP-4; Rat IGFBP-4 (Insulin-Like Growth Factor Binding Protein 4) ELISA Kit; IGFBP-4 elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes Rat IGFBP-1 in samples. No significant cross-reactivity or interference between Rat IGFBP-1 and analogues was observed.
• Sequence Length: 258

• Samples: Serum, Plasma And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 18.75pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IGFBP-1 were tested 20 times on one plate, respectively
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IGFBP-1 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGFBP-4 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat IGFBP-1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IGFBP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IGFBP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IGFBP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat IGFBP-1. You can calculate the concentration of Rat IGFBP-1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 62243290
• NCBI GeneID: 3487
• NCBI Accession #: NP_001543.2
• NCBI GenBank Nucleotide #: NM_001552.2
• UniProt Accession #: P22692; Q5U012; Q9UCL6; A0N9W2; B4E351
• Molecular Weight: 17,635 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 4
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 4
• NCBI Official Symbol: IGFBP4
• NCBI Official Synonym Symbols: BP-4; IBP4; IGFBP-4; HT29-IGFBP
• NCBI Protein Information: insulin-like growth factor-binding protein 4; IBP-4; IGF-binding protein 4
• UniProt Protein Name: Insulin-like growth factor-binding protein 4
• UniProt Gene Name: IGFBP4
• UniProt Synonym Gene Names: IBP4; IBP-4; IGF-binding protein 4; IGFBP-4
• UniProt Entry Name: IBP4_HUMAN

NCBI Description

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]

Uniprot Description

IGFBP4: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: extracellular space; extracellular region

Molecular Function: insulin-like growth factor I binding; insulin-like growth factor II binding

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; cell proliferation; regulation of insulin-like growth factor receptor signaling pathway; cellular protein metabolic process; positive regulation of MAPKKK cascade; regulation of cell growth; inflammatory response; signal transduction; skeletal development; DNA metabolic process