Immunohistochemistry (IHC)
(Caldesmon on Appendix)
Mouse anti-Human Caldesmon Monoclonal Antibody
Caldesmon
Reactivity
Human
Synonyms
Caldesmon; Monoclonal Antibody; anti-Caldesmon antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Form/Format
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Application Notes
1:200
Positive Control
Appendix, Breast
Localization
Cytoplasmic
Principles and Procedures
Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
Preparation and Storage
Store at 2-8 degree C.
To ensure stability, immediately replace vial back in the refrigerator after each use. When stored correctly, the antibody is stable until the expiry date indicated on the label.
To ensure stability, immediately replace vial back in the refrigerator after each use. When stored correctly, the antibody is stable until the expiry date indicated on the label.
Immunohistochemistry (IHC)
(Caldesmon on Appendix)
Immunohistochemistry (IHC)
(Caldesmon on Appendix)
Related Product Information for anti-Caldesmon antibody
Caldesmon is a marker for smooth muscle differentiation. Found in smooth muscle and other tissues, caldesmon interacts with Ca2+-calmodulin, actin, tropomyosin, myosin, and phospholipids. It inhibits the ATPase activity of myosin in smooth muscle, and mediates Ca2+-dependent inhibition of smooth muscle and non-muscle contraction. Caldesmon expression is found in Gastrointestinal Stromal Tumours (GIST), and can be used to differentiate epithelioid mesothelioma from serous papillary carcinoma of the ovary. It is also a specific marker for smooth muscle cells (SMC) and associated neoplasms; therefore, Anti-Caldesmon can be used in the study of the SMC differentiation process as well as the differentiation of other tumours with SMC-like differentiation, including leiomyosarcoma and myofibroblastic tumours.
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Product Notes
The Caldesmon (Catalog #AAA344162) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Caldesmon reacts with Human and may cross-react with other species as described in the data sheet. 1:200. Researchers should empirically determine the suitability of the Caldesmon for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caldesmon, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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