Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Putative peptide YY-3 (PYY3) Standard Curve.)
Human Putative Peptide YY-3 (PYY3) ELISA Kit | PYY3 elisa kit
Human Putative Peptide YY-3 (PYY3) ELISA Kit
Reactivity
Human
Synonyms
Putative Peptide YY-3 (PYY3); Human Putative Peptide YY-3 (PYY3) ELISA Kit; PYY3; RP13-377G1_A.2; PYY3 elisa kit
Reactivity
Human
Specificity
Human Putative peptide YY-3 (PYY3) ELISA Kit has high sensitivity and excellent specificity for detection of Human PYY3. No significant cross-reactivity or interference between Human PYY3 and analogues was observed.
Sequence Length
70
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Putative peptide YY-3 (PYY3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Putative peptide YY-3 (PYY3) Standard Curve.)
Related Product Information for PYY3 elisa kit
Description: This Human Putative peptide YY-3 (PYY3) ELISA Kit employs a two-site sandwich ELISA to quantitate PYY3 in samples. An antibody specific for PYY3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PYY3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYY3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYY3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Peptide YY(3-36), a Y2R agonist, is released from the gastrointestinal tract postprandially in proportion to the calorie content of a meal. PYY(3-36) also inhibits food intake in mice but not in Y2r-null mice, which suggests that the anorectic effect requires the Y2R. Peripheral administration of PYY(3-36) increases c-Fos immunoreactivity in the arcuate nucleus and decreases hypothalamic Npy messenger RNA. Intra-arcuate injection of PYY(3-36) inhibits food intake. PYY(3-36) also inhibits electrical activity of NPY nerve terminals, thus activating adjacent pro-opiomelanocortin (POMC) neurons. In humans, infusion of normal postprandial concentrations of PYY(3-36) significantly decreases appetite and reduces food intake by 33% over 24 h. Thus, postprandial elevation of PYY(3-36) may act through the arcuate nucleus Y2R to inhibit feeding in a gut-hypothalamic pathway.
Background: Peptide YY(3-36), a Y2R agonist, is released from the gastrointestinal tract postprandially in proportion to the calorie content of a meal. PYY(3-36) also inhibits food intake in mice but not in Y2r-null mice, which suggests that the anorectic effect requires the Y2R. Peripheral administration of PYY(3-36) increases c-Fos immunoreactivity in the arcuate nucleus and decreases hypothalamic Npy messenger RNA. Intra-arcuate injection of PYY(3-36) inhibits food intake. PYY(3-36) also inhibits electrical activity of NPY nerve terminals, thus activating adjacent pro-opiomelanocortin (POMC) neurons. In humans, infusion of normal postprandial concentrations of PYY(3-36) significantly decreases appetite and reduces food intake by 33% over 24 h. Thus, postprandial elevation of PYY(3-36) may act through the arcuate nucleus Y2R to inhibit feeding in a gut-hypothalamic pathway.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
NCBI Official Full Name
Putative peptide YY-3
UniProt Protein Name
Putative peptide YY-3
Protein Family
UniProt Gene Name
PYY3
UniProt Synonym Gene Names
Putative peptide YY3
Uniprot Description
PYY3: Belongs to the NPY family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: Xp11.22
Cellular Component: extracellular space
Molecular Function: G-protein coupled receptor binding; neuropeptide hormone activity
Biological Process: cell-cell signaling; digestion; feeding behavior; neuropeptide signaling pathway; regulation of appetite
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Product Notes
The Human PYY3 pyy3 (Catalog #AAA9714676) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714676 ELISA Kit recognizes Human PYY3. It is sometimes possible for the material contained within the vial of "Putative Peptide YY-3 (PYY3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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