Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peptide YY (PYY) Standard Curve.)
Mouse Peptide YY (PYY) ELISA Kit | PYY elisa kit
Mouse Peptide YY (PYY) ELISA Kit
Reactivity
Mouse
Synonyms
Peptide YY (PYY); Mouse Peptide YY (PYY) ELISA Kit; PYY; PYY1; PYY elisa kit
Reactivity
Mouse
Specificity
Mouse Peptide YY (PYY) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PYY. No significant cross-reactivity or interference between Mouse PYY and analogues was observed.
Sequence Length
98
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peptide YY (PYY) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peptide YY (PYY) Standard Curve.)
Related Product Information for PYY elisa kit
Description: This Mouse Peptide YY (PYY) ELISA Kit employs a two-site sandwich ELISA to quantitate PYY in samples. An antibody specific for PYY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PYY present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYY is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYY bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Peptide YY is a short (36 amino acid) protein released by cells in the ileum and colon in response to feeding. It is also known as PYY, Peptide Tyrosine Tyrosine, or Pancreatic Peptide YY3-36. Peptide YY is related to the pancreatic peptide family by having 18 of its 36 amino acids located in the same positions as pancreatic peptide. There are two major forms of Peptide YY: PYY1-36 and PYY3-36 which have PP fold structural motif.However, the most common form of circulating PYY immunoreactivity is PYY3-36 which binds to Y2 receptor (Y2R) of Y family receptors. Peptide YY3-36 (PYY) is a linear polypeptide consisting of 36 amino acids with structural homology to NPY and pancreatic polypeptide.
Background: Peptide YY is a short (36 amino acid) protein released by cells in the ileum and colon in response to feeding. It is also known as PYY, Peptide Tyrosine Tyrosine, or Pancreatic Peptide YY3-36. Peptide YY is related to the pancreatic peptide family by having 18 of its 36 amino acids located in the same positions as pancreatic peptide. There are two major forms of Peptide YY: PYY1-36 and PYY3-36 which have PP fold structural motif.However, the most common form of circulating PYY immunoreactivity is PYY3-36 which binds to Y2 receptor (Y2R) of Y family receptors. Peptide YY3-36 (PYY) is a linear polypeptide consisting of 36 amino acids with structural homology to NPY and pancreatic polypeptide.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
peptide YY isoform 1
NCBI Official Synonym Full Names
peptide YY
NCBI Official Symbol
Pyy
NCBI Protein Information
peptide YY
UniProt Protein Name
Peptide YY
Protein Family
UniProt Gene Name
Pyy
UniProt Synonym Gene Names
PYY
UniProt Entry Name
PYY_MOUSE
Uniprot Description
PYY: This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. Belongs to the NPY family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular space
Molecular Function: G-protein-coupled receptor binding; neuropeptide hormone activity
Biological Process: cell-cell signaling; digestion; eating behavior; negative regulation of response to food; neuropeptide signaling pathway; regulation of appetite
Research Articles on PYY
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Product Notes
The Mouse PYY pyy (Catalog #AAA9718156) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718156 ELISA Kit recognizes Mouse PYY. It is sometimes possible for the material contained within the vial of "Peptide YY (PYY), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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