Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Caltrin (PYY2) Standard Curve.)
Human Caltrin (PYY2) ELISA Kit | PYY2 elisa kit
Human Caltrin (PYY2) ELISA Kit
Gene Names
PYY2; SPLN
Reactivity
Human
Synonyms
Caltrin (PYY2); Human Caltrin (PYY2) ELISA Kit; PYY2 elisa kit
Reactivity
Human
Specificity
Human Caltrin (PYY2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PYY2. No significant cross-reactivity or interference between Human PYY2 and analogues was observed.
Sequence Length
80
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Caltrin (PYY2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Caltrin (PYY2) Standard Curve.)
Related Product Information for PYY2 elisa kit
Description: This Human Caltrin (PYY2) ELISA Kit employs a two-site sandwich ELISA to quantitate PYY2 in samples. An antibody specific for PYY2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PYY2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYY2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYY2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: NPY is an abundant neuropeptide regulating a wide range of physiologic activities. Another member of the NPY family, PYY, is an inhibitory hormone secreted by digestive tract endocrine cells. PPY is a pancreatic hormone inhibiting secretion of enzymes and bicarbonate from the exocrine pancreas. The genes encoding PYY and PPY are thought to have arisen by gene duplication and are localized on chromosome 17q21.1. All 3 genes contain 4 exons with little or no intronic homology. PYY2, like PYY1, has 4 exons and conserved 5-prime and 3-prime flanking sequences as well as conserved introns 2 and 3. Despite 92% identity between the PYY and PYY2 nucleotide sequences, PYY encodes a 98-amino acid protein and PYY2 encodes a 33-residue protein due to an alternative start site, as seen in bovine Pyy2 (seminalplasmin), and an early stop codon.
Background: NPY is an abundant neuropeptide regulating a wide range of physiologic activities. Another member of the NPY family, PYY, is an inhibitory hormone secreted by digestive tract endocrine cells. PPY is a pancreatic hormone inhibiting secretion of enzymes and bicarbonate from the exocrine pancreas. The genes encoding PYY and PPY are thought to have arisen by gene duplication and are localized on chromosome 17q21.1. All 3 genes contain 4 exons with little or no intronic homology. PYY2, like PYY1, has 4 exons and conserved 5-prime and 3-prime flanking sequences as well as conserved introns 2 and 3. Despite 92% identity between the PYY and PYY2 nucleotide sequences, PYY encodes a 98-amino acid protein and PYY2 encodes a 33-residue protein due to an alternative start site, as seen in bovine Pyy2 (seminalplasmin), and an early stop codon.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
caltrin
NCBI Official Symbol
PYY2
NCBI Official Synonym Symbols
SPLN
NCBI Protein Information
caltrin
UniProt Protein Name
Caltrin
Protein Family
UniProt Gene Name
PYY2
UniProt Synonym Gene Names
Peptide YY2; SPLN
UniProt Entry Name
PYY2_BOVIN
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Product Notes
The Human PYY2 pyy2 (Catalog #AAA9714675) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714675 ELISA Kit recognizes Human PYY2. It is sometimes possible for the material contained within the vial of "Caltrin (PYY2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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