Western Blot (WB)
(Western Blot analysis of PRKACB in Mouse testis tissue lysate using PRKACB Rabbit pAb at dilution 1/300)
Rabbit PRKACB Polyclonal Antibody | anti-PRKACB antibody
PRKACB Rabbit pAb
IHC-P: 1:50-1:100
Western Blot (WB)
(Western Blot analysis of PRKACB in Mouse testis tissue lysate using PRKACB Rabbit pAb at dilution 1/300)
Western Blot (WB)
(Western Blot analysis of PRKACB in Mouse testis tissue lysate using PRKACB Rabbit pAb at dilution 1/300)
Immunohistochemistry (IHC)
(Immunohistochemistry of PRKACB in paraffin-embedded Human tonsil tissue using PRKACB Rabbit pAb at dilution 1/50. Predicted cell location: Cytoplasm)
Immunohistochemistry (IHC)
(Immunohistochemistry of PRKACB in paraffin-embedded Human tonsil tissue using PRKACB Rabbit pAb at dilution 1/50. Predicted cell location: Cytoplasm)
NCBI and Uniprot Product Information
NCBI Description
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Several alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jun 2011]
Uniprot Description
PKACB: Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. The cAMP-dependent protein kinase catalytic subunit binds PJA2. Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain. Activated by cAMP. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. 9 isoforms of the human protein are produced by alternative splicing.
Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; EC 2.7.11.11; AGC group; PKA family
Chromosomal Location of Human Ortholog: 1p31.1
Cellular Component: nucleoplasm; centrosome; perinuclear region of cytoplasm; plasma membrane; cytosol; cAMP-dependent protein kinase complex
Molecular Function: protein binding; cAMP-dependent protein kinase activity; ubiquitin protein ligase binding; magnesium ion binding; ATP binding
Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; activation of protein kinase A; glucose metabolic process; negative regulation of meiotic cell cycle; pathogenesis; signal transduction; protein amino acid phosphorylation; gluconeogenesis; G-protein signaling, coupled to cAMP nucleotide second messenger; synaptic transmission; phospholipase C activation; neural tube closure; carbohydrate metabolic process; triacylglycerol catabolic process; energy reserve metabolic process; innate immune response; renal water homeostasis; blood coagulation; regulation of insulin secretion; transmembrane transport
Research Articles on PRKACB
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Product Notes
The PRKACB prkacb (Catalog #AAA4759875) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRKACB Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PRKACB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000 IHC-P: 1:50-1:100. Researchers should empirically determine the suitability of the PRKACB prkacb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRKACB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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