Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 (ACAP1) Standard Curve.)
Human Arf-GAP With Coiled-Coil, ANK Repeat And PH Domain-Containing Protein 1 (ACAP1) ELISA Kit | ACAP1 elisa kit
Human Arf-GAP With Coiled-Coil, ANK Repeat And PH Domain-Containing Protein 1 (ACAP1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 (ACAP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 (ACAP1) Standard Curve.)
Background: ACAP1 and ACAP2 were recruited to platelet-derived growth factor (PDGF)-induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, and overexpression inhibited ruffle formation. The deduced protein contains 740 amino acids and shares 52% sequence identity with CENTB2. highest expression in spleen, thymus, and peripheral blood leukocytes, intermediate expression in lung, testis, and small intestine, and weak expression in prostate, ovary, and colon. No expression was detected in heart, brain, placenta, liver, skeletal muscle, kidney, and pancreas, and no expression was detected in HeLa cells.ACAP1 shares significant similarity with CENTB2, which the authors called ACAP2, and 95% identity with mouse Acap1.
NCBI and Uniprot Product Information
Uniprot Description
CENTB1: a member of the centaurin family of ADP-ribosylation factor directed GTPase-activating proteins (GAPs). A GAP for ARF1 and ARF5. Two alternatively-spliced isoforms have been described. Isoform 1 is brain-specific, and isoform 2 is ubiquitously expressed, with highest levels in brain and heart. Isoform 1 participates to the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. Regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. The PH domains of isoforms 1 and 2 bind phospholipids, but they differentially regulate subcellular location. The PH domain of isoform 1 directs the protein to the nucleus, but the PH domain of isoform 2 directs it to the cytosol.
Protein type: Motility/polarity/chemotaxis; GAPs, ARF; GAPs
Chromosomal Location of Human Ortholog: 17p13.1
Cellular Component: membrane; recycling endosome membrane
Molecular Function: zinc ion binding
Biological Process: protein transport; positive regulation of GTPase activity
Research Articles on ACAP1
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Product Notes
The Human ACAP1 acap1 (Catalog #AAA9714538) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714538 ELISA Kit recognizes Human ACAP1. It is sometimes possible for the material contained within the vial of "Arf-GAP With Coiled-Coil, ANK Repeat And PH Domain-Containing Protein 1 (ACAP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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