Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Transformer-2 protein homolog beta (TRA2B) Standard Curve.)
Human Transformer-2 Protein Homolog beta (TRA2B) ELISA Kit | TRA2B elisa kit
Human Transformer-2 Protein Homolog beta (TRA2B) ELISA Kit
Gene Names
TRA2B; SFRS10
Reactivity
Human
Synonyms
Transformer-2 Protein Homolog beta (TRA2B); Human Transformer-2 Protein Homolog beta (TRA2B) ELISA Kit; TRA2B; DKFZp686F18120; Htra2-beta; SFRS10; SRFS10; TRA2-BETA; TRAN2B; splicing factor; arginine/serine-rich 10; arginine/serine-rich 10 (transformer 2 homolog; Drosophila); transformer 2 hom; TRA2B elisa kit
Reactivity
Human
Specificity
Human Transformer-2 protein homolog beta (TRA2B) ELISA Kit has high sensitivity and excellent specificity for detection of Human TRA2B. No significant cross-reactivity or interference between Human TRA2B and analogues was observed.
Sequence Length
288
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Transformer-2 protein homolog beta (TRA2B) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Transformer-2 protein homolog beta (TRA2B) Standard Curve.)
Related Product Information for TRA2B elisa kit
Description: This Human Transformer-2 protein homolog beta (TRA2B) ELISA Kit employs a two-site sandwich ELISA to quantitate TRA2B in samples. An antibody specific for TRA2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRA2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRA2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRA2B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TRA2-beta gene contains 10 exons and spans 21 kb. RT-PCR and analysis of TRA2-beta cDNAs in the EST database indicated that 5 different TRA2-beta RNA isoforms are generated by alternative splicing. TRA2-beta-1 is a nuclear protein that colocalizes with SC35 in a speckled pattern. It interacts with several SR proteins in yeast. A second isoform, named TRA2-beta-2, is generated by alternative splicing. This isoform gives rise to a truncated protein without an SR domain. Both isoforms are evenly distributed throughout adult rat tissue. The ratio of these 2 isoforms changes after stimulation of primary human T-cell and primary rat spleen cell cultures, indicating that alternative splicing is involved in the regulation of TRA2-beta activity.
Background: TRA2-beta gene contains 10 exons and spans 21 kb. RT-PCR and analysis of TRA2-beta cDNAs in the EST database indicated that 5 different TRA2-beta RNA isoforms are generated by alternative splicing. TRA2-beta-1 is a nuclear protein that colocalizes with SC35 in a speckled pattern. It interacts with several SR proteins in yeast. A second isoform, named TRA2-beta-2, is generated by alternative splicing. This isoform gives rise to a truncated protein without an SR domain. Both isoforms are evenly distributed throughout adult rat tissue. The ratio of these 2 isoforms changes after stimulation of primary human T-cell and primary rat spleen cell cultures, indicating that alternative splicing is involved in the regulation of TRA2-beta activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
transformer-2 protein homolog beta
NCBI Official Synonym Full Names
transformer 2 beta homolog
NCBI Official Symbol
TRA2B
NCBI Official Synonym Symbols
SFRS10
NCBI Protein Information
transformer-2 protein homolog beta
UniProt Protein Name
Transformer-2 protein homolog beta
Protein Family
UniProt Gene Name
TRA2B
UniProt Synonym Gene Names
SFRS10; TRA-2 beta; TRA2-beta
UniProt Entry Name
TRA2B_BOVIN
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Product Notes
The Human TRA2B tra2b (Catalog #AAA9713809) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713809 ELISA Kit recognizes Human TRA2B. It is sometimes possible for the material contained within the vial of "Transformer-2 Protein Homolog beta (TRA2B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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