Human Transformer 2 Beta (TRA2b) ELISA Kit | TRA2b elisa kit

Human Transformer 2 Beta (TRA2b) ELISA Kit

Cat. Num. AAA2707078

• Gene Names: TRA2B; SFRS10; SRFS10; TRAN2B; PPP1R156; TRA2-BETA; Htra2-beta
• Reactivity: Human
• Synonyms: Transformer 2 Beta (TRA2b); Human Transformer 2 Beta (TRA2b) ELISA Kit; SRFS10; Htra2-beta; TRA2-BETA; TRA2B; Splicing Factor; Arginine/Serine-Rich 10; Transformer-2 protein homolog B; TRA2b elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transformer 2 Beta (TRA2b).; No significant cross-reactivity or interference between Transformer 2 Beta (TRA2b) and analogues was observed.
• Sequence Length: 188

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transformer 2 Beta (TRA2b) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transformer 2 Beta (TRA2b) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TRA2b elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transformer 2 Beta (TRA2b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transformer 2 Beta (TRA2b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transformer 2 Beta (TRA2b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transformer 2 Beta (TRA2b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1553035
• NCBI GeneID: 6434
• Molecular Weight: 21,935 Da
• NCBI Official Full Name: transformer-2 beta
• NCBI Official Synonym Full Names: transformer 2 beta homolog
• NCBI Official Symbol: TRA2B
• NCBI Official Synonym Symbols: SFRS10; SRFS10; TRAN2B; PPP1R156; TRA2-BETA; Htra2-beta
• NCBI Protein Information: transformer-2 protein homolog beta
• UniProt Protein Name: Transformer-2 protein homolog beta
• Protein Family: Transformer-2 protein
• UniProt Gene Name: TRA2B
• UniProt Synonym Gene Names: SFRS10; TRA-2 beta; TRA2-beta; hTRA2-beta

NCBI Description

This gene encodes a nuclear protein which functions as sequence-specific serine/arginine splicing factor which plays a role in mRNA processing, splicing patterns, and gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Uniprot Description

Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA.